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Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad f...
Montoya-Serrano, Francisco S.; Dal Vesco, Lírio L.; Pescador, RoseteVriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentage, germination speed index (GSI), and total soluble carbohydrate...
DNA methylation patterns and karyotype analysis of off-type and normal phenotyp...
Cristofolini, Caroline; Vieira, Leila do Nascimento; Fraga, Hugo Pacheco de Freitas; Costa, Itayguara Ribeiro da; Guerra, Miguel Pedro; Pescador, RoseteAcca sellowiana is a native species to the southern region of Brazil, with secondary dispersion in Uruguay northeast. The potentially economic interest of this species motivated many fundamental and applied researches, such as micropropagation protocols development, including somatic embryogenesis (SE). Previous studies have reported the high frequency of off-type somatic embryos in A. sellowiana SE and low con...
Assestement of plant growth regulation properties of indole composites derived ...
Mengarda, Liana Hilda Golin; Pescador, Rosete; da Rosa, Flavia Aparecida Fernandes; Rebelo, Ricardo AndradeThe auxin properties of new indole composites synthetized from safrole 2-AMIM and 3-AMIM were evaluated by means of assays in vitro, in which leaf explants of P. hispidinervium, submitted to different treatments, were analyzed as to the presence and number of roots and shoots, callus formation, intumescence and oxidation. The treatments suplemented with 3-AIA, as well as those with 2-AMIM and 3-AMIM, were less ...
Dynamics of proteins, carbohydrates and global DNA methylation patterns during ...
Corredor, Jenny; De Conti, Daniela; Guerra, Miguel Pedro; Dal Vesco, Lirio Luiz; Pescador, RoseteTissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) t...
Determination of endogenous IAA and carbohydrates during the induction and deve...
De Conti, Daniela; Corredor-Prado, Jenny Paola; Roecker Junior, Djalma; Suzuki, Rogério Mamoru; Guerra, Miguel Pedro; Pescador, RoseteTissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indole-acetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Le...
Fertility and reserves analysis in buds of ‘Greco di Tufo’, ‘Coda di Volpe’ and...
Munhoz, Bruno; Welter, Juliana Fátima; Pescador, Rosete; Brighenti, Alberto Fontanella; Silva, Aparecido Lima daABSTRACT – Fertility, total soluble carbohydrates and starch contents were evaluated in dormant buds of ‘Greco di Tufo’, ‘Coda di Volpe’ and ‘Viognier’ grapevines in an experimental vineyard located in São Joaquim (SC), in 2013. Buds from one year, deep dormant shoots, were observed in 3 positions: basal buds (1st to 3rd bud), medians buds (4th to 6th bud) and apical buds (7th to 10th bud). The presence or abse...
Dynamics in global DNA methylation and endogenous polyamine levels during proto...
Almeida, Vivian; Fraga, Hugo Pacheco de Freitas; Navarro, Bruno Bachiega; Guerra, Miguel Pedro; Pescador, RoseteCattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with tha...
Estado físico do meio de cultura na propagação in vitro de bromeliaceae
Mengarda, Liana Hilda Golin; Povoas, Luciano; Debiasi, Clayton [UNESP]; Pescador, RoseteSubmitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:24:02Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:30:26Z : No. of bitstreams: 1 2-s2.0-84883291868.pdf: 363228 bytes, checksum: e1cb8835253d8969d75fa45ff3a3cfb4 (MD5); Made available in DSpace on 2014-05-27T11:24:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-11-01; The objective of this study was to ...
MORFO-HISTOLOGIA DE CULTURAS NODULARES NA MICROPROPAGAÇÃO DE Aechmea setigera M...
Fermino Junior, Paulo Cesar Poeta; Lando, Ana Paula; Santos, Marisa; Pescador, RoseteAechmea setigera é uma espécie de Bromeliaceae da Amazônia Ocidental com potencial ornamental. A micropropagação por culturas nodulares consiste em uma estratégia de propagação massal, as quais apresentam alto potencial regenerativo, culminando na produção múltipla de brotos adventícios em condições de cultura adequadas. O objetivo deste trabalho foi induzir a formação de culturas nodulares a partir de folhas j...
In vitro propagation of Vriesea reitzii, a native epiphyte bromeliad from the A...
Dal Vesco, Lírio Luiz; Pescador, Rosete; Prado, Jenny Paola Corredor; Welter, Leocir José; Guerra, Miguel PedroThe induction of nodular culture (NC) and the subsequent development of microshoots of V. reitzii are considered an in vitro propagation model-system with high regenerative performance. Current research analyzed the determinant factors of the in vitro morphogenesis control of bromeliads. Seeds excised from mature capsules were grown on medium MS basic (MSB), liquid or gelled, supplemented or not with α-naphthal...