9 documents found, page 1 of 1

Role of fibrinogen–erythrocyte and erythrocyte–erythrocyte adhesion on cardiova...

Fibrinogen–erythrocyte binding and hemorheology measurements in the assessment ...

Some studies have reported a positive association between plasma fibrinogen levels, erythrocyte aggregation and essential arterial hypertension (EAH). The aim of this study was to understand how the interaction between fibrinogen and its erythrocyte membrane receptor is altered in EAH. EAH patients (n = 31) and healthy blood donors (n = 65) were enrolled in the study. EAH patients were therapeutically controlle...

Prognostic stratification of adult primary glioblastoma multiforme patients bas...

Several classification systems have been proposed to address genomic heterogeneity of glioblastoma multiforme, but they either showed limited prognostic value and/or are difficult to implement in routine diagnostics. Here we propose a prognostic stratification model for these primary tumors based on tumor gene amplification profiles, that might be easily implemented in routine diagnostics, and potentially impro...

Molecular characterization of the fibrinogen-erythrocyte interaction as a cardi...

Cardiovascular diseases (CVD) are the major cause of death worldwide. The evaluation of new risk factors can help to identify individuals at higher risk. Erythrocyte aggregation is one of the indicators of cardiovascular risk, which seems to be influenced by high plasma fibrinogen levels. This acute plasma protein has an alternatively spliced γchain variant (γ’ fibrinogen) that has been particularly associated ...

Impact of γ’γ’ fibrinogen interaction with red blood cells on fibrin clots

AIM: γ' fibrinogen has been associated with thrombosis. Here the interactions between γ'γ' or γAγA fibrinogen and red blood cells (RBCs), and their role on fibrin clot properties were studied. MATERIALS & METHODS: Atomic Force microscopy (AFM)-based force spectroscopy, rheological, electron and confocal microscopy, and computational approaches were conducted for both fibrinogen variants. RESULTS & CONCLUSION: A...

The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to ...

BACKGROUND: Erythrocyte aggregation, a cardiovascular risk factor, is increased by high plasma fibrinogen levels. Here, the effect of different fibrinogen mutations on binding to its human erythrocyte receptor was assessed in order to identify the interaction sites. METHODS: Three fibrinogen variants were tested, specifically mutated in their putative integrin recognition sites on the Aα chain (mutants D97E, D5...

Sensing adhesion forces between erythrocytes and γ’ fibrinogen, modulating fibr...

Plasma fibrinogen includes an alternatively spliced γ-chain variant (γ'), which mainly exists as a heterodimer (γAγ') and has been associated with thrombosis. We tested γAγ' fibrinogen-red blood cells (RBCs) interaction using atomic force microscopy-based force spectroscopy, magnetic tweezers, fibrin clot permeability, scanning electron microscopy and laser scanning confocal microscopy. Data reveal higher work ...

Atomic force microscopy as a tool to evaluate the risk of cardiovascular diseas...

The availability of biomarkers to evaluate the risk of cardiovascular diseases is limited. High fibrinogen levels have been identified as a relevant cardiovascular risk factor, but the biological mechanisms remain unclear. Increased aggregation of erythrocytes (red blood cells) has been linked to high plasma fibrinogen concentration. Here, we show, using atomic force microscopy, that the interaction between fib...

Atomic force microscopy and graph analysis to study the P-cadherin/SFK mechanot...

Physical forces mediated by cell–cell adhesion molecules, as cadherins, play a crucial role in preserving normal tissue architecture. Accordingly, altered cadherins’ expression has been documented as a common event during cancer progression. However, in most studies, no data exist linking protumorigenic signaling and variations in the mechanical balance mediated by adhesive forces. In breast cancer, P-cadherin ...