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Stunning and slaughter methods in gilthead seabream: animal welfare and muscle ...

Cabrera-Álvarez, María José; Soares, Sónia Marina António; Nuñez Velazquez, Samira; Anibal, Jaime; Esteves, Eduardo; Costa, Rita

European Union directives and international guidelines emphasise the need for humane slaughter practices, with particular attention to the proper stunning of fish prior to slaughter. This study investigates the impact of various combinations of stunning and slaughter methods on the welfare of gilthead seabream (Sparus aurata), a widely farmed species in the Mediterranean and Black Sea regions. Specifically, the...


Off the shelf: regulation of fish blood pH through ionocyte membrane remodellin...

Guerreiro, Pedro Miguel; Canario, Adelino

Ocean acidification (OA), together with warming and deoxygenation, forms the “deadly trio,” a combination that reduces ecosystems’ resilience, makes marine biodiversity vulnerable, and is considered a main contributor to global mass extinctions in the Earth’s history (1).


Insights into conservation success: analysing shore angling before implementing...

Jacinto, Gonçalo; Veiga, Pedro; Monteiro, Pedro; Erzini, Karim; Gonçalves, Jorge Manuel Santos; Horta e Costa, Barbara; Pontes, João

In January 2024, the "Natural Marine Park of the Algarve Reef - Pedra do Valado" (NMPAR-PV; southern Portugal), a community-based Marine Protected Area (MPA) was formally classified, setting the scene for a new paradigm for co-creating Portuguese MPAs. While complying with the best practices for MPA establishment, this area also contributes to national and international conservation targets. Measuring the succe...


Combined effects of PVC microplastics and thermal rise alter the oxidative stre...

Nualart, Daniela P.; Paschke, Kurt; Guerreiro, Pedro Miguel; McCormick, Stephen D.; González-Wevar, Claudio; Cheng, Chi-Hing Christina

This study evaluated the oxidative stress response in two cold-water fish species, the Antarctic Harpagifer antarcticus and the sub-Antarctic H. bispinis, following exposure to single and combined stressors: polyvinyl chloride (PVC) microplastics and thermal increase. Fish were exposed for 24 h to two temperature regimes (2 degrees C and 5 degrees C for H. antarcticus; 8 degrees C and 12 degrees C for H. bispin...


The immune system in antarctic and subantarctic fish of the genus harpagifer is...

Nualart, Daniela P.; Guerreiro, Pedro Miguel; Paschke, Kurt; McCormick, Stephen D.; Cheng, Chi-Hing Christina; Vargas-Chacoff, Luis

Rising ocean temperatures due to climate change, combined with the intensification of anthropogenic activity, may lead to changes in the physiology and distribution of native species. Compounding climate stress, microplastic particles (MPs) enter the oceans through wastewater and the breakdown of macroplastics. Depending on their composition, they can be harmful and act as a vehicle for toxic substances, althou...


Assessing fish welfare in small-scale commercial fixed-net fisheries off the so...

Samel, Vighnesh Nilesh; Costa, Rita; Marçalo, Ana; Frade, Magda; Bentes, Luis; Saraiva, João; Gonçalves, Jorge Manuel Santos; Guerreiro, Pedro Miguel

Despite a growing interest in animal welfare in production systems, research on fish welfare remains limited, particularly in commercial fisheries. Fish caught in fixed-net fisheries experience multiple stressors from the time of capture to mortality on deck considered detrimental to their welfare. We examined the impact of bottom-set gill nets and on-board handling on catch welfare using behavioural and physio...


Anatomy of the olfactory system and potential role for chemical communication i...

Modesto, Teresa; Gregório, Beatriz Neves; Marcelino, Gonçalo; Marquet, Nathalie; Costa, Rita; Guerreiro, Pedro Miguel; Velez, Zélia; Hubbard, Peter

The current study investigated the structure and function of the olfactory system of the Lusitanian toadfish, Halobatrachus didactylus, using histology and electrophysiology (electro-olfactogram [EOG]), respectively. The olfactory system consists of a digitated anterior peduncle, of unknown function, containing the inhalant nostril. This then leads to a U-shaped olfactory chamber with the olfactory epithelium-i...


A comparison of olfactory sensitivity in seawater- and freshwater-adapted bass,...

Velez, Zélia; Hubbard, Peter; Guerreiro, Pedro Miguel

Fish rely heavily on olfaction for many aspects of their lives including foraging, defense, migration, and reproduction. Olfactory receptor neurons in the olfactory epithelium are in direct contact with the water, and are, therefore, exposed to changes in water chemistry. The European seabass, Dicentrarchus labrax, uses estuaries as feeding grounds and migrates between seawater and brackish water; but some can ...


Sperm characterization of endangered leuciscids endemic from the iberian penins...

Hernandez, Ana; Sousa-Santos, Carla; Gil, Fátima; Guerreiro, Pedro Miguel; Cabrita, Elsa; Gallego, Victor

Populations of freshwater fish species endemic to the Iberian Peninsula have been declining since the mid-20th century, and several types of actions (from in situ to ex situ measurements) have been applied over the past decades for preserving these species. However, limited knowledge about their reproductive biology makes it necessary to investigate different aspects of the reproductive cycle for improving bree...


Transcriptomic analysis of differentially expressed genes in kidney and intesti...

Vargas Lagos, Carolina Fabiola; Silva, Sandra; Guerrero, Laura; Oca, Marco Montes de; Louro, Bruno; Carvalho Alves, Alexandra; Rotllant, Josep

Phosphorus (P), in the form of inorganic phosphate (Pi), is one of the most important macronutrients for all organisms, including fish. It is indispensable for the formation of hard tissues such as bones, but also for cell signalling and cell membrane formation, and energy transduction, among many other functions and is kept under well-controlled conditions, since its deficiency or overload may lead to skeletal...


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