5 documents found, page 1 of 1
A dual-function SNF2 protein drives chromatid resolution and nascent transcript...
Carmo, Catarina; Coelho, João; Silva, Rui D.; Tavares, Alexandra; Boavida, Ana; Gaetani, Paola; Guilgur, Leonardo G.; Martinho, Rui GonçaloMitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs a...
Absence of the spindle assembly checkpoint restores mitotic fidelity upon loss ...
Silva, Rui; Mirkovic, Mihailo; Guilgur, Leonardo G.; Rathore, Om; Martinho, Rui Goncalo; Oliveira, Raquel A.The fidelity of mitosis depends on cohesive forces that keep sister chromatids together. This is mediated by cohesin that embraces sister chromatid fibers from the time of their replication until the subsequent mitosis [1-3]. Cleavage of cohesin marks anaphase onset, where single chromatids are dragged to the poles by the mitotic spindle [4-6]. Cohesin cleavage should only occur when all chromosomes are properl...
Early programming of the oocyte epigenome temporally controls late prophase I t...
Navarro-Costa, Paulo; McCarthy, Alicia; Prudêncio, Pedro; Greer, Christina; Guilgur, Leonardo G.; Becker, Jörg D.; Secombe, Julie; Rangan, PrashanthOocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here w...
Drosophila aPKC is required for mitotic spindle orientation during symmetric di...
Guilgur, Leonardo G.; Prudencio, Pedro; Ferreira, Tania; Pimenta-Marques, Ana Rita; Goncalo Martinho, RuiEpithelial cells mostly orient the spindle along the plane of the epithelium (planar orientation) for mitosis to produce two identical daughter cells. The correct orientation of the spindle relies on the interaction between cortical polarity components and astral microtubules. Recent studies in mammalian tissue culture cells suggest that the apically localised atypical protein kinase C (aPKC) is important for t...
Evolution of GnRH ligands and receptors in gnathostomata
Guilgur, Leonardo G.; Moncaut, Natalia P.; Canario, Adelino V. M.; Somoza, G. M.Gonadotropin-releasing hormone (GnRH) is the final common signaling molecule used by the brain to regulate reproduction in all vertebrates. Until now, a total of 24 GnRH structural variants have been characterized from vertebrate, protochordate and invertebrate nervous tissue. Almost all vertebrates already investigated have at least two GnRH forms coexisting in the central nervous system. Furthermore, it is no...