17 documents found, page 1 of 2
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to humans. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers, some chemically induced DNA adducts. Depending on the specimen type, there ar...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Moller, Peter; Gajski, Goran; Vodenkova, Sona; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...
Invasive clams (Ruditapes philippinarum) are better equipped to deal with harmf...
Braga, Ana C.; Marcal, Raquel; Marques, Ana; Guilherme, Sofia; Vilarino, Oscar; Martins, J. Manuel Leao; Gago-Martinez, Ana; Reis Costa, PedroThis study aims to assess and compare the kinetics (accumulation/elimination) of the marine biotoxins okadaic acid (OA) and dinophysistoxin-1 (DTX1 ), between native (Ruditapes decussates) and invasive (Ruditapes philippinarum) clam species, and their genotoxic effects and DNA recover capacity after, exposure to toxic dinoflagellates Promcentrum lima. Clams were fed with P. lima for 5 days and then to non-toxic...
DNA damage and oxidative stress responses of mussels Mytilus galloprovincialis ...
Braga, Ana C.; Pereira, Vitória; Marçal, Raquel; Marques, Ana; Guilherme, Sofia; Costa, Pedro R.; Pacheco, MárioCommonly affected by changes in climate and environmental conditions, coastal areas are very dynamic environments where shellfish play an important ecological role. In this study, the oxidative stress and genotoxic responses of mussels (Mytilus galloprovincialis) exposed to paralytic shellfish toxin (PST) - producing dinoflagellates Gymnodinium catenatum were evaluated under i) current conditions (CC: 19 °C; pH...
DNA damage and oxidative stress responses of mussels Mytilus galloprovincialis ...
Braga, Ana C.; Pereira, Vitoria; Marcal, Raquel; Marques, Ana; Guilherme, Sofia; Reis Costa, Pedro; Pacheco, MarioCommonly affected by changes in climate and environmental conditions, coastal areas are very dynamic environments where shellfish play an important ecological role. In this study, the oxidative stress and genotoxic responses of mussels (Mytilus galloprovincialis) exposed to paralytic shellfish toxin (PST) - producing dinoflagellates Gymnodinium catenatum were evaluated under i) current conditions (CC: 19 degree...
Marine macroalgae as a dietary source of genoprotection in gilthead seabream (S...
Pereira, Vitória; Marques, Ana; Gaivão, Isabel; Rego, Andreia; Abreu, Helena; Pereira, Rui; Santos, Maria Ana; Guilherme, Sofia; Pacheco, MárioDNA integrity and stability are essential to organisms' health and survival. However, it has been neglected in what concerns to fish farming, disregarding the potential impact of endogenous/ exogenous factors. As marine macroalgae constitute a source of natural compounds with a large spectrum of biological activities, this study, situated in the interface of nutritional-genetic research and development of algae...
Metals(loids) targeting fish eyes and brain in a contaminated estuary - Uncover...
Pereira, Ricardo; Leite, Eduarda; Raimundo, Joana; Guilherme, Sofia; Puga, Sónia Andreia Silva; Pinto-Ribeiro, Filipa; Santos, Maria Ana; Canário, JoãoThis study examined the susceptibility of fish (Liza aurata) eyes and brain to metals(loids) contamination under realistic exposure conditions. A multidimensional approach was applied to fish caught at a chronically contaminated site (BAR) and at a reference site of the Tagus estuary (Portugal), which comprised metals(loids) accumulation in eyes and brain together with a battery of enzymatic and non-enzymatic a...
Metals(loids) targeting fish eyes and brain in a contaminated estuary - uncover...
Pereira, Ricardo; Leite, Eduarda; Raimundo, Joana; Guilherme, Sofia; Puga, Sónia; Pinto-Ribeiro, Filipa; Santos, Maria Ana; Canário, JoãoThis study examined the susceptibility of fish (Liza aurata) eyes and brain to metals(loids) contamination under realistic exposure conditions. A multidimensional approach was applied to fish caught at a chronically contaminated site (BAR) and at a reference site of the Tagus estuary (Portugal), which comprised metals(loids) accumulation in eyes and brain together with a battery of enzymatic and non-enzymatic a...
Progression of DNA damage induced by a glyphosate-based herbicide in fish (Angu...
Marques, Ana; Guilherme, Sofia; Gaivão, Isabel; Santos, Maria Ana; Pacheco, MárioRoundup(R) is a glyphosate-based herbicide widely used with both agricultural and non-agricultural purposes, which has been demonstrated to represent a risk to non-target aquatic organisms, namely fish. Among the described effects to fish, genotoxicity has been pointed out as one of the most hazardous. However, the genotoxic mechanisms of Roundup?? as well as the involvement of the oxidative DNA damage repair s...