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Precise multiphase hydrogel engineering of miniaturized 3D cancer architectures...
Rial, Ramón; Guimarães, Carlos Ferreira; Gasperini, Luca; Brito, Alexandra; Costa, Rui R.; Ruso, Juan M.; Reis, R. L.Understanding cancer biology and therapy responses requires accurate in vitro models that reflect tumor complexity. This work presents a multiphase microfluidic biofabrication approach for creating self-standing three-dimensional (3D) tumor models within hydrogel microfiber boundaries. A single framework enabled the fast generation of different in vitro cellular configurations, including discrete spheroids ...
Cu+/Ca2+ Dual‐overload strategy assisted by metabolic‐symbiotic‐destruction for...
Yan, Jiahui; Chen, Haoyu; Guimarães, Carlos Ferreira; Reis, R. L.; Zhu, Zhihua; Wang, Tianyi; Zhang, Fenglan; Zhou, Qihui; Kong, Xiaoying; Shi, JinshengIon interference including copper (Cu+)/calcium (Ca2+) overload activate cell-specific death channels, damage mitochondria and disrupt cellular homeostasis, showing great potential in anti-tumor therapy. However, the complex metabolic environment and the powerful self-protection of tumors cause clinical failure of ion interference. Thus, metabolic disruption is expected an innovative strategy for the enhancemen...
Co-axial hydrogel spinning for facile biofabrication of prostate cancer-like 3D...
Guimarães, Carlos Ferreira; Liu, Shiqin; Wang, Jie; Purcell, Emma; Ozedirne, Tugba; Ren, Tanchen; Aslan, Merve; Yin, Qingqing; Reis, R. L.Glandular cancers are amongst the most prevalent types of cancer, which can develop in many different organs, presenting challenges in their detection as well as high treatment variability and failure rates. For that purpose, anticancer drugs are commonly tested in cancer cell lines grown in 2D tissue culture on plastic dishes in vitro, or in animal models in vivo. However, 2D culture models diverge significant...
Activated immune system determines in vivo anti-tumor effect of phototherapy by...
Ding, Mengchao; Chen, Haoyu; Guimarães, Carlos Ferreira; Reis, R. L.; Wu, Lijuan; Zhang, Fenglan; Lv, Yaqian; Wang, Tianyi; Zhou, Qihui; Shi, JinshengImmune activation has been widely recognized to promote anti-tumor effects in vivo. However, in previous anti-tumor studies of combined photo/immunotherapy, the tumor apoptosis pathway is widely regarded as an independent process, which led to an incomplete understanding of the anti-tumor mechanisms of the immune system during phototherapy. Herein, the decisive role of immune activation for the anti-tumor effec...
Microfluidic hydrogel biofabrication for high-throughput screening, modeling an...
Guimarães, Carlos FerreiraThe functionality of living tissues depends entirely on their intricate 3D composition, architecture, and mechanics, which result from the complex interplay between cells and ECM. Correctly approaching these characteristics will be fundamental to engineer next-generation platforms for in vitro modeling of healthy and diseased tissues. Further, the capacity to miniaturize 3D microenvironments for faster composit...
Shining a light on cancer - photonics in microfluidic tumor modeling and biosen...
Guimarães, Carlos Ferreira; Cruz-Moreira, Daniela; Caballero, David; Pirraco, Rogério P.; Gasperini, Luca; Kundu, Subhas C; Reis, R. L.Microfluidic platforms represent a powerful approach to miniaturizing important characteristics of cancers, improving in vitro testing by increasing physiological relevance. Different tools can manipulate cells and materials at the microscale, but few offer the efficiency and versatility of light and optical technologies. Moreover, light-driven technologies englobe a broad toolbox for quantifying critical biolo...
Large-Scale functionalized metasurface-based SARS-CoV-2 detection and quantific...
Ahmed, R.; Guimarães, Carlos Ferreira; Wang, J.; Soto, F.; Karim, A. H.; Zhang, Z.; Reis, R. L.; Akin, D.; Paulmurugan, R.; Demirci, U.Plasmonic metasurfaces consist of metal–dielectric interfaces that are excitable at background and leakage resonant modes. The sharp and plasmonic excitation profile of metal-free electrons on metasurfaces at the nanoscale can be used for practical applications in diverse fields, including optoelectronics, energy harvesting, and biosensing. Currently, Fano resonant metasurface fabrication processes for biosenso...