15 documents found, page 1 of 2
The Barretos Cancer Hospital Animal Facility: implementation and results of a d...
Teixeira, Silvia A.; Luzzi, Mayara de Cassia; Martin, Ana Carolina Baptista Moreno; Duarte, Terence Teixeira; Leal, Mônica de OliveiraThe Barretos Cancer Hospital Animal Facility (BCHAF) is a unique facility in Brazil exclusively dedicated to working with animal models for cancer research. In this article, we briefly present our modern facility and the main experiments performed, focusing on mutant strains of mice (PTCH-knockout and <i>Apc<sup>Min</sup></i> mice), xenograft models, and patient-derived xenografts (PDXs). Our results show the p...
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associate...
Munari, Fernanda Franco; Sichero, Laura; Carloni, Adriana Cruvinel; Lacerda, Croider Franco; Nunes, Emily Montosa; De Oliveira, Antônio Talvane TorresMade available in DSpace on 2022-04-29T08:35:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01; Background: Chagasic megaesophagus (CM) as well as the presence of human papillomavirus (HPV) has been reported as etiological factors for esophageal squamous cell carcinoma (ESCC). Objective: We assessed the prevalence of HPV DNA in a series of ESCCs associated or not with CM. Data obtained were furthe...
Mutation profling of cancer drivers in Brazilian colorectal cancer
Santos, Wellington dos; Sobanski, Thais; Carvalho, Ana Carolina de; Evangelista, Adriane Feijó; Matsushita, Marcus; Berardinelli, Gustavo NórizThe molecular basis of colorectal cancer (CRC) can guide patient prognosis and therapy. In Brazil, knowledge on the CRC mutation landscape is limited. Here, we investigated the mutation profile of 150 cancer-related genes by next-generation sequencing and associated with microsatellite instability (MSI) and genetic ancestry in a series of 91 Brazilian CRC patients. Driver mutations were found in the APC (71.4%)...
PIK3CA mutations are frequent in esophageal squamous cell carcinoma associated ...
Munari, Fernanda Franco; Cruvinel-Carloni, Adriana; Lacerda, Croider Franco; De Oliveira, Antônio Talvane Torres; Scapulatempo-Neto, CristovamMade available in DSpace on 2019-10-06T16:11:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-29; Background: Chronic diseases such as chagasic megaesophagus (secondary to Chagas' disease) have been suggested as etiological factors for esophageal squamous cell carcinoma; however, the molecular mechanisms involved are poorly understood. Objective: We analyzed hotspot PIK3CA gene mutations in a series...
Microbiota profile and impact of fusobacterium nucleatum in colorectal cancer p...
de Carvalho, Ana Carolina; de Mattos Pereira, Leandro; Datorre, José Guilherme; Dos Santos, Wellington; Berardinelli, Gustavo NorizMicrobial diversity has been pointed out as a major factor in the development and progression of colorectal cancer (CRC). We sought to explore the richness and abundance of the microbial community of a series of colorectal tumor samples treated at Barretos Cancer Hospital, Brazil, through 16S rRNA sequencing. The presence and the impact of Fusobacterium nucleatum (Fn) DNA in CRC prognosis was further evaluated ...
Prevalence of high risk HPV DNA in esophagus is high in Brazil but not related ...
Costa, Allini Mafra da; Fregnani, José Humberto Tavares Guerreiro; Pastrez, Paula Roberta Aguiar; Mariano, Vânia SammartinoThe first publication that associated Human Papillomavirus (HPV) infection and esophageal cancer was published in 1982. However, data are still contradictory and require further investigation. The aim of this study was to identify high risk HPV DNA in esophageal tissue of patients with and without esophageal squamous cell carcinoma (ESCC) and correlate HPV presence with classical risk factors. METHODS: Invited ...
PIK3CA mutations are frequent in esophageal squamous cell carcinoma associated ...
Munari, Fernanda Franco; Cruvinel-Carloni, Adriana; Lacerda, Croider Franco; de Oliveira, Antônio Talvane Torres; Scapulatempo-Neto, CristovamChronic diseases such as chagasic megaesophagus (secondary to Chagas' disease) have been suggested as etiological factors for esophageal squamous cell carcinoma; however, the molecular mechanisms involved are poorly understood.
The relation of HPV infection and expression of p53 and p16 proteins in esophag...
Pastrez, Paula Roberta Aguiar; Mariano, Vânia Sammartino; Costa, Allini Mafra da; Silva, Estela Maria; Scapulatempo-Neto, CristovamGOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). RESULTS: High-risk HPV (hr-HPV) wa...
Lack of microsatellite instability in gastrointestinal stromal tumors
Campanella, Nathália C.; Scapulatempo-Neto, Cristovam; Abrahão-Machado, Lucas Faria; Oliveira, Antônio Talvane Torres; Berardinelli, Gustavo N.The microsatellite instability (MSI) phenotype may constitute an important biomarker for patient response to immunotherapy, particularly to anti-programmed death-1 inhibitors. MSI is a type of genomic instability caused by a defect in DNA mismatch repair (MMR) proteins, which is present mainly in colorectal cancer and its hereditary form, hereditary nonpolyposis colorectal cancer. Gastrointestinal stromal tumor...
HPV infection and p53 and p16 expression in esophageal cancer: are they prognos...
Costa, Allini Mafra da; Fregnani, José Humberto Tavares Guerreiro; Pastrez, Paula Roberta Aguiar; Mariano, Vânia Sammartino; Silva, Estela MariaBackground: Esophageal squamous cell carcinoma (ESCC) is a highly lethal malignant tumor. Currently, Human papillomavirus (HPV) is suggested as a potential risk factor for esophageal cancer (EC) in addition to the classic risk factors, alcohol and tobacco, but this hypothesis still remains contradictory. We sought to investigate wether HPV and well-known biomarkers (p16 and p53) and patient-related factors that...