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Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci...
Queiroz, Paulo Roberto; Martins, Erica Soares; Guimarães, Maria de Nazaré Klautau; Lima, Luzia; Praça, Lilian; Monnerat, Rose GomesO objetivo deste trabalho foi desenvolver marcadores "sequence-characterized amplified region" (Scar) para identificar os biótipos B, Q e nativo brasileiro da mosca-branca [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Produtos de amplificação de DNA polimórfico amplificado ao acaso (RAPD), exclusivos aos biótipos B e nativo brasileiro, foram selecionados após análise de 12.000 amostras, para desenhar um conjunto d...
Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transf...
Hiragi, Cássia de Oliveira; Miranda-Vilela, Ana Luisa; Rocha, Dulce Maria Sucena; Oliveira, Silviene Fabiana de; Hatagima, AnaAntioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX1) reduce the oxidation rates in the organism. Gluthatione S-transferases (GSTs) play a vital role in phase 2 of biotransformation of many substances. Variation in the expression of these enzymes suggests individual differences for the degree of antioxidant protection and geographical differences in the distribution o...
. Acesso em: 24 out. 2017. doi: http://dx.doi.org/10.1590/S1415-47572010005000022." title="3. Dietary carotenoid-rich oil supplementation improves exercise-induced anisocytosis in runners : influences of haptoglobin, MnSOD (Val9Ala), CAT (21A/T) and GPX1 (Pro198Leu) gene polymorphisms in dilutional pseudoanemia ("sports anemia")">Dietary carotenoid-rich oil supplementation improves exercise-induced anisocyto...
Vilela, Ana Luísa Miranda; Akimoto, Arthur Kenji; Alves, Penha Cristina Zaidan; Pereira, Luiz Carlos da Silva; Guimarães, Maria de Nazaré KlautauPhysical training induces beneficial adaptation, whereas exhaustive exercises increase reactive oxygen-species generation, thereby causing oxidative damage in plasma and erythrocytes, fractions susceptible to lipid peroxidation. Pequi (Caryocar brasiliense Camb.) is a Brazilian Cerrado fruit containing a carotenoid-rich oil. The aim was to investigate the effects of pequi-oil on exercise-induced oxidative damag...
β-globin haplotypes in normal and hemoglobinopathic individuals from Reconcavo ...
Silva, Wellington dos Santos; Guimarães, Maria de Nazaré Klautau; Grisolia, Cesar KoppeFive restriction site polymorphisms in the β-globin gene cluster (HincII-5'ε, HindIII-Gγ, HindIII-ªγ, HincII-'ψβ1 and HincII-3''ψβ1) were analyzed in three populations (n = 114) from Reconcavo Baiano, State of Bahia, Brazil. The groups included two urban populations from the towns of Cachoeira and Maragojipe and one rural Afro-descendant population, known as the "quilombo community", from Cachoeira municipality...
Avaliação da cobertura do programa de triagem neonatal de hemoglobinopatias em ...
Silva, Wellington dos Santos; Lastra, Angel; Oliveira, Silviene Fabiana de; Guimarães, Maria de Nazaré Klautau; Grisólia, César KoppeEm 2001, a Portaria n. 822/2001 do Ministério da Saúde tornou obrigatória a triagem neonatal para as hemoglobinopatias, especialmente a anemia falciforme, a doença genética mais comum no Brasil. A Bahia, em decorrência de sua história de povoamento, é o Estado com maior prevalência dessa doença no país. No presente trabalho, apresentamos a cobertura da triagem neonatal (número de recém-nascidos/número de triage...
Distribution of glutathione S-transferase GSTM1 and GSTT1 null phenotypes in Br...
Guimarães, Maria de Nazaré Klautau; Hiragi, Cássia de Oliveira; D'Ascenção, Renata Ferreira; Oliveira, Silviene Fabiana de; Grisolia, Cesar KoppeThe distribution of glutathione S-transferase (GST) GSTM1 and GSTT1 null phenotype frequencies in two Brazilian Amerindian tribes, the Munduruku tribe from Missão Cururu village (79 individuals) and the Kayabi tribe (41 individuals), was analyzed by polymerase chain reaction (PCR) amplification. The GST null phenotype frequencies for the Munduruku sample were 0% for GSTM1 and 27% for GSTT1 while for the Kayabi ...
Glutathione S-transferase M1 (GSTM1) polymorphism in two Brazilian populations
Hatagima, Ana; Guimarães, Maria de Nazaré Klautau; Silva, Felizardo Penalva da; Cabello, Pedro HernanA distribuição das freqüências fenotípicas do gene GSTM1 de duas amostras brasileiras, compostas de doadores sanguíneos de ambos os sexos com idade entre 18 e 61 anos, foi estudada. A primeira amostra é composta de 658 indivíduos provenientes do Rio de Janeiro e a segunda de 179 indivíduos de Brasília. Os fenótipos da GSTM1 foram determinados utilizando-se a técnica da reação em cadeia da polimerase e subsequen...
Haptoglobin gene subtypes in three Brazilian population groups of different eth...
Vilela, Ana Luísa Miranda; Akimoto, Arthur Kenji; Alves, Penha Cristina Zaidan; Hiragi, Cássia de Oliveira; Penalva, Guilherme C.; Oliveira, Silviene F.Haptoglobin is a plasma hemoglobin-binding protein that limits iron loss during normal erythrocyte turnover and hemolysis, thereby preventing oxidative damage mediated by iron excess in the circulation. Haptoglobin polymorphism in humans, characterized by the Hp*1 and Hp*2 alleles, results in distinct phenotypes known as Hp1-1, Hp2-1 and Hp2-2, whose frequencies vary according to the ethnic origin of the popula...
Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment ...
Grisolia, Cesar Koppe; Oliveira, Adriano Barros Barata de; Bonfim, Helder; Guimarães, Maria de Nazaré KlautauAn evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochromis niloticus and Tilapia rendalli, through the index of micronuclei in peripheral erythrocytes. In the Allium test...
Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocyte...
Miranda-Vilela, Ana Luisa; Alves, Penha Cristina Zaidan; Akimoto, Arthur K.; Lordelo, Graciana S.; Gonçalves, Carlos A.; Grisólia, César KoppeBackground: Normal cellular metabolism is well established as the source of endogenous reactive oxygen species which account for the background levels of oxidative DNA damage detected in normal tissue. Hydrogen peroxide imposes an oxidative stress condition on cells that can result in DNA damage, leading to mutagenesis and cell death. Several potentially significant genetic variants related to oxidative stress ...