5 documents found, page 1 of 1
First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a...
Rozental,Tatiana; Faria,Letícia Scafutto De; Forneas,Danielle; Guterres,Alexandro; Ribeiro,João Batista; Araújo,Flábio RibeiroABSTRACT Background: Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Ob...
Hemoparasites in ticks of wild birds of Serra dos Órgãos National Park, state o...
Pacheco,Adlilton; Cordeiro,Matheus Dias; Cepeda,Marcio Barizon; Luz,Hermes Ribeiro; Cardozo,Sergian Vianna; Berto,Bruno Pereira; Guterres,AlexandroAbstract The aim of this study is to detect the presence of tick-borne agents of genera Rickettsia, Borrelia, Babesia, Ehrlichia and Anaplasma in ticks collected from native wild birds in the state of Rio de Janeiro. Birds were captured and observed carefully to find the ectoparasites. DNA detection of hemoparasites was performed by means of the polymerase chain reaction (PCR). The sequences obtained were analy...
Clinical and epidemiological use of nested PCR targeting the repetitive element...
Mares-Guia,Maria Angélica M.M.; Guterres,Alexandro; Rozental,Tatiana; Ferreira,Michelle dos Santos; Lemos,Elba R.S.ABSTRACT Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of...
Morphological, molecular and phylogenetic characterization of Borrelia theileri...
Cordeiro,Matheus Dias; Bahia,Michele; Magalhães-Matos,Paulo Cesar; Cepeda,Marcio Barizon; Guterres,Alexandro; Fonseca,Adivaldo HenriqueAbstract This study aimed to perform a morphological, molecular and phylogenetic characterization of Borrelia theileri obtained from infected Rhipicephalus microplus in Brazil. Fifty engorged R. microplus females from cattle in the municipality of Seropédica, Rio de Janeiro, were analyzed for spirochetes by hemolymph smear. Macerated eggs and positive ticks, as well as blood from the bovine infested by these ti...
Characterization of rickettsia rickettsii in a case of Fatal Brazilian spotted ...
Lamas,Cristiane; Favacho,Alexsandra; Rozental,Tatiana; Bóia,Márcio N.; Kirsten,Andrei H.; Guterres,Alexandro; Barreira,Jairo; Lemos,Elba Regina S. deA lethal case of Brazilian spotted fever (BSF) is presented. Clinical features were initially of gastrointestinal involvement and evolved with progression to septic shock, meningoencephalitis and death on the 6th day of illness. Indirect immunofluorescence assay (IFA) for spotted fever group rickettsia (SFGR) was non-reactive. Diagnosis was confirmed by the polymerase chain reaction (PCR) and the nucleotide seq...