4 documents found, page 1 of 1
The hCOMET project: International database comparison of results with the comet...
Milić, M; Ceppi, M; Bruzzone, M; Azqueta, A; Brunborg, G; Godschalk, R; Koppen, G; Langie, S; Møller, P; Teixeira, JP; Alija, A; Anderson, D; Andrade, VThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent...
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations f...
Møller, P; Azqueta, A; Boutet-Robinet, E; Koppen, G; Bonassi, S; Milić, M; Gajski, G; Costa, S; Teixeira, JP; Costa Pereira, C; Dusinska, MThe comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here...
The comet assay in animal models: From bugs to whales - (Part 2 Vertebrates)
Gajski, G; Žegura, B; Ladeira, C; Novak, M; Sramkova, M; Pourrut, B; Del Bo', C; Milić, M; Gutzkow, KB; Costa, S; Dusinska, M; Brunborg, G; Collins, AThe comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the...
The comet assay in animal models: From bugs to whales - (Part 1 Invertebrates)
Gajski, G; Žegura, B; Ladeira, C; Pourrut, B; Del Bo', C; Novak, M; Sramkova, M; Milić, M; Gutzkow, KB; Costa, S; Dusinska, M; Brunborg, G; Collins, AThe comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of...