16 documents found, page 1 of 2
First report of two consecutive respiratory syncytial virus outbreaks by the no...
Silva,Daniella Gregoria Bomfim Prado da; Almeida,Flávia Jacqueline; Arnoni,Mariana Volpe; Sáfadi,Marco Aurélio Palazzi; Mimica,Marcelo JenneAbstract Objective: Respiratory syncytial virus is a pathogen frequently involved in nosocomial outbreaks. Although several studies have reported nosocomial outbreaks in neonatal intensive care units, molecular epidemiology data are scarce. Here, the authors describe two consecutive respiratory syncytial virus outbreaks caused by genotypes ON-1 and NA-2 in a neonatal intensive care unit in São Paulo, Brazil. Me...
Rapid antigen detection test for respiratory syncytial virus diagnosis as a dia...
Mesquita,Flávio da Silva; Oliveira,Danielle Bruna Leal de; Crema,Daniela; Pinez,Célia Miranda Nunes; Colmanetti,Thaís CristinaAbstract Objective: The aim of this study was to evaluate the QuickVue® RSV Test Kit (QUIDEL Corp, CA, USA) as a screening tool for respiratory syncytial virus in children with acute respiratory disease in comparison with the indirect immunofluorescence assay as gold standard. In Brazil, rapid antigen detection tests for respiratory syncytial virus are not routinely utilized as a diagnostic tool, except for the...
Survey of pathogens in threatened wild red-tailed Amazon parrot (Amazona brasil...
Vaz,Frederico Fontanelli; Serafini,Patrícia Pereira; Locatelli-Dittrich,Rosangela; Meurer,Rafael; Durigon,Edison Luiz; Araújo,Jansen deABSTRACT The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and...
Prenatal diagnosis of congenital rubella infection in São Paulo
Curti,Suely Pires; Figueiredo,Cristina Adelaide; Oliveira,Maria Isabel de; Andrade,Joelma Queiroz; Zugaib,Marcelo; Pedreira,Denise Araújo LapaObjective: rubella during the early stages of pregnancy can lead to severe birth defects known as congenital rubella syndrome (CRS). Samples collected from pregnant women with symptoms and suspected of congenital rubella infection between 1996 and 2008 were analyzed. Methods: a total of 23 amniotic fluid samples, 16 fetal blood samples, 1 product of conception and 1 placenta were analyzed by serology and RT-PCR...
Seasonality of viral respiratory infections in Southeast of Brazil: the influen...
Gardinassi,Luiz Gustavo; Simas,Paulo Vitor Marques; Salomão,João Batista; Durigon,Edison Luiz; Trevisan,Dirce Maria Zanetta; Cordeiro,José AntonioViruses are the major cause of lower respiratory tract infections in childhood and the main viruses involved are Human Respiratory Syncytial Virus (HRSV), Human Metapneumovirus (HMPV), Influenzavirus A and B (FLUA and FLUB), Human Parainfluenza Virus 1, 2 and 3 (HPIV1, 2 and 3) and Human Rhinovirus (HRV). The purposes of this study were to detect respiratory viruses in hospitalized children younger than six yea...
Gravidade das coinfecções virais em lactentes hospitalizados com infecção por v...
Paulis,Milena De; Gilio,Alfredo Elias; Ferraro,Alexandre Archanjo; Ferronato,Angela Esposito; Sacramento,Patrícia Rossi do; Botosso,Viviane FongaroOBJETIVO: Comparar a gravidade de infecções causadas por um único vírus (VSR) com a gravidade de coinfecções. MÉTODOS: Este estudo avaliou uma coorte histórica de lactentes com infecção aguda por VSR. Secreção de nasofaringe foi coletada de todos os pacientes rotineiramente para pesquisa viral usando técnicas de biologia molecular. Os seguintes desfechos foram analisados: tempo total de internação, duração da o...
Molecular characterization of rabies virus isolated from non-haematophagous bat...
Albas,Avelino; Campos,Angélica Cristine de Almeida; Araujo,Danielle Bastos; Rodrigues,Camila Seabra; Sodré,Miriam Martos; Durigon,Edison LuizINTRODUCTION: Rabies is an important zoonosis that causes thousands of deaths worldwide each year. Although the terrestrial cycle, mainly transmitted by dogs, is controlled in Brazil, the aerial cycle remains a serious public health issue, besides the economic problem. In the aerial cycle, the haematophagous bat Desmodus rotundus is the main source of infection, where several different species of non-haematopha...
First report of rabies infection in bats, Molossus molossus, Molossops neglectu...
Rosa,Adriana Ruckert da; Kataoka,Ana Paula de Arruda Geraldes; Favoretto,Silvana Regina; Sodré,Miriam Martos; Trezza Netto,JoséINTRODUCTION: This paper presents the first report of rabies in three bat species, Molossus molossus, Molossops neglectus and Myotis riparius in the city of São Paulo, Brazil. METHODS: Bats were diagnosed as positive for rabies using the fluorescent antibody test and mouse inoculation test. The isolates were characterized antigenically using a panel of eight monoclonal antibodies. The samples were also genetica...
Comparison between ectodomain and G2 region of G glycoprotein for genotyping of...
Campos,Angélica Cristine de Almeida; Durigon,Edison Luiz; Leal,Andréa Lima; Silva,Tereza Souza; Bosso,Patrícia Alves Ramos; Moraes,Claudia Trigo PedrosoThe Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparati...
Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
Carnieli Junior,Pedro; Ventura,Armando Moraes; Durigon,Edison LuizA digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this techniq...