3 documents found, page 1 of 1
An anaphase surveillance mechanism prevents micronuclei formation from frequent...
Orr, B; Sousa, F; Gomes, AM; Afonso, O; Ferreira, LT; Figueiredo, AC; Maiato, HMicronuclei are a hallmark of cancer and several other human disorders. Recently, micronuclei were implicated in chromothripsis, a series of massive genomic rearrangements that may drive tumor evolution and progression. Here, we show that Aurora B kinase mediates a surveillance mechanism that integrates error correction during anaphase with spatial control of nuclear envelope reassembly to prevent micronuclei f...
Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 cros...
Afonso, O; Castellani, CM; Cheeseman, LP; Ferreira, JG; Orr, B; Ferreira, LT; Chambers, JJ; Morais-de-Sá, E; Maresca, TJ; Maiato, HAccording to the prevailing ‘clock’ model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at the metaphase-anaphase transition, controlled by the spindle assembly checkpoint. However, mitotic exit was recently shown to be a function of chromosome separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient-the ‘ruler’...
Feedback control of chromosome separation by a midzone Aurora B gradient
Afonso, O; Matos, I; Pereira, AJ; Aguiar, P; Lampson, MA; Maiato, HAccurate chromosome segregation during mitosis requires the physical separation of sister chromatids before nuclear envelope reassembly (NER). However, how these two processes are coordinated remains unknown. Here, we identified a conserved feedback control mechanism that delays chromosome decondensation and NER in response to incomplete chromosome separation during anaphase. A midzone-associated Aurora B gradi...