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Molecular Detection of Metronidazole and Tetracycline Resistance Genes in Helic...

Nunes, FC; Taillieu, E; Mateus, TL; Teixeira, S; Haesebrouck, F; Amorim, I

Antimicrobial resistance is a major public health concern. The aim of this study was to assess the presence of antibiotic resistance genes, previously reported in Helicobacter pylori, in gastric samples of 36 pigs, in which DNA of H. pylori-like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the 16S rRNA mutation gene, conferring tetracycline resistance, and one...


Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs a...

Cortez Nunes, F; Letra, Mateus, T; Taillieu, E; Teixeira, S; Carolino, N; Rema, A; De Bruyckere, S; Gärtner, F; Haesebrouck, F; Amorim, I

Besides Helicobacter pylori, a Gram-negative bacterium that may cause gastric disorders in humans, non-Helicobacter pylori helicobacters (NHPH) may also colonize the stomach of humans and animals. In pigs, H. suis can induce gastritis and may play a role in gastric ulcer disease, possibly in association with Fusobacterium gastrosuis. In the present study, gastric samples from 71 slaughtered pigs and 14 hunted f...


Helicobacter spp. in the Stomach of Cats: Successful Colonization and Absence o...

Teixeira, S; Filipe, D; Cerqueira, M; Barradas, P; Nunes, FC; Faria, F; Haesebrouck, F; Mesquita, JR; Gärtner, F; Amorim, I

In addition to Helicobacter pylori, many non-Helicobacter pylori Helicobacters (NHPH) are able to cause gastric disease in humans. Cats are a natural reservoir for many of these species. Accordingly, living in close and intimate contact with animals has been identified as a risk factor, and an important zoonotic significance has therefore been attributed to NHPH. To determine the prevalence and associated gastr...


Presence of Helicobacter pylori and H. suis DNA in Free-Range Wild Boars

Cortez Nunes, F; Letra Mateus, T; Teixeira, S; Barradas, P; de Witte, C; Haesebrouck, F; Amorim, I; Gärtner, F

Helicobacter pylori (H. pylori) is a Gram-negative bacterium that infects half of the human population worldwide, causing gastric disorders, such as chronic gastritis, gastric or duodenal ulcers, and gastric malignancies. Helicobacter suis (H. suis) is mainly associated with pigs, but can also colonize the stomach of humans, resulting in gastric pathologies. In pigs, H. suis can induce gastritis and seems to pl...


Adhesion of Helicobacter Species to the Human Gastric Mucosa: A Deep Look Into ...

Matos, R; Amorim, I; Magalhães, A; Haesebrouck, F; Gärtner, F; Reis, CA

Helicobacter species infections may be associated with the development of gastric disorders, such as gastritis, peptic ulcers, intestinal metaplasia, dysplasia and gastric carcinoma. Binding of these bacteria to the gastric mucosa occurs through the recognition of specific glycan receptors expressed by the host epithelial cells. This review addresses the state of the art knowledge on these host glycan structure...


Antimicrobial susceptibility pattern of Helicobacter heilmannii and Helicobacte...

Matos, R; Witte, C; Smet, A; Berlamont, H; Bruyckere, S; Amorim, I; Gärtner, F; Haesebrouck, F

A combined agar and broth dilution method followed by qPCR was used to determine the antimicrobial susceptibility of feline H. heilmannii and H. ailurogastricus isolates. All H. ailurogastricus isolates showed a monomodal distribution of MICs for all the antimicrobial agents tested. For H. heilmannii, a bimodal distribution was observed for azithromycin, enrofloxacin, spectinomycin, and lincomycin. Single nucle...


A comparison of Helicobacter pylori and non-Helicobacter pylori Helicobacter sp...

Amorim, I; Freitas, DP; Magalhães, A; Faria, F; Lopes, C; Faustino, A; Smet, A; Haesebrouck, F; Reis, CA; Gärtner, F

Background: The gastric mucosa of dogs is often colonized by non-Helicobacter pylori helicobacters (NHPH), while H. pylori is the predominant gastric Helicobacter species in humans. The colonization of the human gastric mucosa by H. pylori is highly dependent on the recognition of host glycan receptors. Our goal was to define the canine gastric mucosa glycophenotype and to evaluate the capacity of different gas...


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