3 documents found, page 1 of 1
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slid...
Brustoloni, Yvone Maia; Lima, Rosimar Batista; Cunha, Rivaldo Venâncio da; Dorval, Maria Elizabeth; Oshiro, Elisa Teruya; Oliveira, Ana Lúcia Lyrio deThe aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with...
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of vi...
Fraga, Thiago Leite; Brustoloni, Yvone Maia; Lima, Rosimar Baptista; Dorval, Maria Elizabeth Cavalheiros; Oshiro, Elisa Teruya; Oliveira, JanainaThe diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peri...
Leishmaniose visceral em crianças no estado de Mato Grosso do Sul, Brasil : con...
Brustoloni, Yvone MaiaTese (doutorado)—Universidade de Brasília, Faculdade de Medicina, 2006.; A faixa etária pediátrica é especialmente atingida pela leishmaniose visceral (LV), uma doença atualmente em franca expansão no Mato Grosso do Sul e em outras localidades brasileiras. O diagnóstico e o tratamento da LV em crianças foram estudados retrospectivamente, através da análise dos dados de pacientes internados no Hospital Universit...