3 documents found, page 1 of 1
Detection of Rickettsia in ticks using loop-mediated isothermal amplification (...
Lansdell, Samantha; Hassan, Marwa M.; La Ragione, Roberto; Betson, Martha; Nuncio, MS; Lopes de Carvalho, Isabel; Zé-Zé, Líbia; de Sousa, RitaObjectives: The objective of the study was to develop a Loop-mediated Isothermal Amplification (LAMP) assay for screening of Rickettsia species circulating in ticks using the citrate synthase gene (gltA). The LAMP assay employed portable visualisation methods, making the assay more field-suitable. Furthermore, prior methods have not used gltA as the target, despite proven success in Polymerase Chain Reaction (P...
Portable Differential Detection of CTX-M ESBL Gene Variants, blaCTX-M-1 and bla...
Higgins, Owen; Chueiri, Alexandra; O'Connor, Louise; Lahiff, Sinéad; Burke, Liam; Morris, Dearbhaile; Pfeifer, Nicola Maria; Santamarina, Belén GonzálezCefotaximase-Munich (CTX-M) extended-spectrum beta-lactamase (ESBL) enzymes produced by Enterobacteriaceae confer resistance to clinically relevant third-generation cephalosporins. CTX-M group 1 variants, CTX-M-1 and CTX-M-15, are the leading ESBL-producing Enterobacteriaceae associated with animal and human infection, respectively, and are an increasing antimicrobial resistance (AMR) global health concern. The...
Mapping the evidence of the effects of environmental factors on the prevalence ...
Gardner, Brian; Betson, Martha; Cabal Rosel, Adriana; Caniça, Manuela; Chambers, Mark A.; Contadini, Francesca M.; Gonzalez Villeta, Laura C.Background: Human, animal, and environmental health are increasingly threatened by the emergence and spread of antibiotic resistance. Inappropriate use of antibiotic treatments commonly contributes to this threat, but it is also becoming apparent that multiple, interconnected environmental factors can play a significant role. Thus, a One Health approach is required for a comprehensive understanding of the envir...