2 documents found, page 1 of 1
Staphylococci phages display vast genomic diversity and evolutionary relationships
Oliveira, Hugo; Sampaio, Marta; Melo, Luís Daniel Rodrigues; Dias, Oscar; Pope, Welkin H.; Hatfull, Graham F.; Azeredo, JoanaBacteriophages are the most abundant and diverse entities in the biosphere, and this diversity is driven by constant predator--prey evolutionary dynamics and horizontal gene transfer. Phage genome sequences are under-sampled and therefore present an untapped and uncharacterized source of genetic diversity, typically characterized by highly mosaic genomes and no universal genes. To better understand the diversit...
Application of BRED technology to construct recombinant D29 reporter phage expr...
Silva, Joas L. da; Piuri, Mariana; Broussard, Gregory; Marinelli, Laura J.; Bastos, Gisele M.; Hirata, Rosario D.C.; Hatfull, Graham F.Bacteriophage Recombineering of Electroporated DNA (BRED) has been described for construction of gene deletion and point mutations in mycobacteriophages. Using BRED, we inserted a Phsp60-egfp cassette (1143 bp) into the mycobacteriophage D29 genome to construct a new reporter phage, which was used for detection of mycobacterial cells. The cassette was successfully inserted and recombinant mycobacteriophage puri...