26 documents found, page 1 of 3

Synthesis, In Vitro Biological Evaluation of Antiproliferative and Neuroprotect...

Steroids constitute an important class of pharmacologically active molecules, playing key roles in human physiology. Within this group, 16E-arylideneandrostane derivatives have been reported as potent anti-cancer agents for the treatment of leukemia, breast and prostate cancers, and brain tumors. Additionally, 5α,6α-epoxycholesterol is an oxysterol with several biological activities, including regulation of cel...

Author Correction: Efficient spatially targeted gene editing using a near-infra...

Saliva for diabetes’ complications: non-invasive diagnostics and monitoring

Type 2 Diabetes Mellitus is a chronic metabolic disease that poses a significant long-term health risk due to its debilitating complications. While the diagnosis and monitoring of diabetes are well-established, the monitoring of its numerous complications presents a challenge, which can compromise patients' prognosis and quality of life. We analyzed the proteome of ten saliva samples, five from healthy individu...

Efficient spatially targeted gene editing using a near-infrared activatable pro...

Spatial control of gene expression is critical to modulate cellular functions and deconstruct the function of individual genes in biological processes. Light-responsive gene-editing formulations have been recently developed; however, they have shown limited applicability in vivo due to poor tissue penetration, limited cellular transfection and the difficulty in evaluating the activity of the edited cells. Here,...

COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 ...

COVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics and in silico interactomics stra...

Advances and challenges in retinoid delivery systems in regenerative and therap...

Retinoids regulate a wide spectrum of cellular functions from the embryo throughout adulthood, including cell differentiation, metabolic regulation, and inflammation. These traits make retinoids very attractive molecules for medical purposes. In light of some of the physicochemical limitations of retinoids, the development of drug delivery systems offers several advantages for clinical translation of retinoid-b...

MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiatio...

There is a high quest for novel therapeutic strategies to enhance recovery after stroke. MicroRNA-124 (miR-124) has been described as neuroprotective and anti-inflammatory molecule. Moreover, miR-124 is a well described enhancer of adult neurogenesis that could offer potentially beneficial effects. Herein, we used miR-124-loaded nanoparticles (miR-124 NPs) to evaluate their therapeutic potential in an in vitro ...

Anti-Inflammatory Strategy for M2 Microglial Polarization Using Retinoic Acid-L...

Inflammatory mechanisms triggered by microglial cells are involved in the pathophysiology of several brain disorders, hindering repair. Herein, we propose the use of retinoic acid-loaded polymeric nanoparticles (RA-NP) as a means to modulate microglia response towards an anti-inflammatory and neuroprotective phenotype (M2). RA-NP were first confirmed to be internalized by N9 microglial cells; nanoparticles did ...

Microglia in Health and Disease: A Double-Edged Sword

Blue light potentiates neurogenesis induced by retinoic acid-loaded responsive ...

Preprint version; The authors would like to thank Ana Clara Cristóvão for her contributions. This work was funded by Fundação para a Ciência e Tecnologia (FCT), FEDER and COMPETE; (PTDC/SAU-NEU/104415/2008, FCOMP-01-0124-FEDER-041099, SFRH/BD/79526/2011), L'Oréal-UNESCO Portugal for Women in Science, Professor Francisco Pulido Valente Foundation, EC (ERC project no. 307384, “Nanotrigger”), COMPETE (Centro-07-ST...