3 documents found, page 1 of 1
Size controlled protein nanoemulsions for active targeting of folate receptor p...
Loureiro, Ana Isabel Sá; Nogueira, Eugénia Sofia Costa; Azóia, Nuno G.; Sárria, M. P.; Abreu, Ana S.; Shimanovich, Ulyana; Rollett, AlexandraBovine Serum Albumin (BSA) nanoemulsions were produced by high pressure homogenization with a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size - the use of 5 mg/mL of Poloxamer 407 yields nanoemulsi...
Peptide anchor for folate-targeted liposomal delivery
Nogueira, E.; Mangialavori, Irene C.; Loureiro, Ana; Azóia, Nuno G.; Sarria, Marisa P.; Nogueira, Patrícia; Freitas, Jaime; Harmark, JohanSpecific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fra...
Liposome and protein based stealth nanoparticles
Nogueira, Eugénia Sofia Costa; Loureiro, Ana; Nogueira, Patrícia; Freitas, Jaime; Almeida, Catarina R.; Harmark, Johan; Hebert, Hans; Moreira, AlexandraLiposomes and protein based nanoparticles were tuned with different polymers and glycolipids to improve stealth and thus decrease their clearance by macrophages. Liposomes were coated with polyethylene glycol (PEG) and brain-tissue-derived monosialoganglioside (GM1). Bovine serum albumin (BSA) nanoparticles were produced incorporating a PEGylated surfactant (PEG-surfactant). All obtained nanoparticles were mono...