14 documents found, page 1 of 2
Selection and characterization of peptides mimetic to Campylobacter fetus subsp...
Ribeiro,Eduardo Lara; Fujimura,Patrícia Tiemi; Ueira-Vieira,Carlos; Goulart,Luiz Ricardo; Alves,Telma Maria; Lage,Andrey Pereira; Heinemann,Marcos BryanABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections ...
Seroprevalence and incidence of Leptospira spp. in domestic dogs in the Southea...
Sevá,Anaiá P.; Brandão,Ana Pérola D.; Godoy,Silvia N.; Souza,Gisele O.; Souza Filho,Antonio Francisco; Jimenez-Villegas,Tatiana; Heinemann,Marcos BryanABSTRACT: Leptospirosis is a zoonotic disease caused by Leptospira and domestic dogs can act as host of some serovars. In order to analyze the transmission dynamics in a dog population, with and without immunization, a longitudinal study was carried out with a focus to evaluate antibody response and to identify serovars. Blood samples were collected in three consecutive years (2015 to 2017) from 331, 373 and 34...
Virulence factors and phylotyping of Escherichia coli isolated from non-diarrhe...
Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria SuhetABSTRACT: This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One ...
Isolation and identification of Mycobacterium bovis in bovines with positive re...
Ramos,Joelson Marcolino; Heinemann,Marcos Bryan; Ferreira Neto,José Soares; Souza Filho,Antonio Francisco de; Cárdenas,Nicolás CéspedesABSTRACT: In areas where human tuberculosis and bovine tuberculosis coexist, differentiation between M. bovis and M. tuberculosis is important for monitoring the spread of M. bovis among cattle and from cattle to humans. The objective of this study was to isolate and identify M. bovis in bovines with positive diagnosis identified on tuberculin test in the State of Paraíba, Northeastern Brazil. Thirty-two bovine...
Detection of virulence genes and the phylogenetic groups of Escherichia coli is...
Coura,Fernanda Morcatti; Diniz,Amanda Nadia; Oliveira Junior,Carlos Augusto; Lage,Andrey Pereira; Lobato,Francisco Carlos Faria; Heinemann,Marcos BryanABSTRACT: This study identified the virulence genes, pathovars, and phylogenetic groups of Escherichia coli strains obtained from the feces of dogs with and without diarrhea. Virulence genes and phylogenetic group identification were studied using polymerase chain reaction. Thirty-seven E. coli isolates were positive for at least one virulence factor gene. Twenty-one (57.8%) of the positive isolates were isolat...
Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius i...
Scherer,Carolina Boesel; Botoni,Larissa Silveira; Coura,Fernanda Morcatti; Silva,Rodrigo Otávio; Santos,Rafael Dantas dos; Heinemann,Marcos BryanABSTRACT: Infections by Staphyloccocus pseudintermedius in the skin of dogs have been object of studies, since such microorganisms often present multiple resistance to antibiotics. This study aimed to identify and evaluate the antimicrobial susceptibility of Staphylococcus pseudintermedius (SP) strains isolated from dogs with otitis. Swabs from both ears of 52 dogs with otitis (n=104) were included. Bacteria we...
Development and validation of a modified TaqMan based real-time PCR assay targe...
Miotto,Bruno Alonso; Hora,Aline Santana da; Taniwaki,Sueli Akemi; Brandão,Paulo Eduardo; Heinemann,Marcos Bryan; Hagiwara,Mitika KuribayashiAbstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were compared ...
Different methods of real-time PCR for detection of pseudorabies virus
Nonaka,Carolina Kymie Vasques; Fonseca Junior,Antônio Augusto; Guedes,Estefânia Oliveira; D´Ambros,Régia Maria; Lima,Graciela KunrathABSTRACT: Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine industry. The aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the molecular diagnosis of PR. Eight primer pairs targeting four genes (gB, gC, gE, gD), three different qPCR chemistries (SybrGreen, hydrolysis probes and plexor) and two eq...
Isolation and identification of Mycobacterium bovis in milk from cows in northe...
Ramos,Joelson Marcolino; Heinemann,Marcos Bryan; Ferreira Neto,José Soares; Filho,Antônio Francisco de Souza; Cárdenas,Nicolás CéspedesABSTRACT: Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraíba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as belonging to the Mycobacterium genus. This is probably the first study of isolation and identification of M...
Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally ...
Oliveira,Andre Penido; Heinemann,Marcos Bryan; Cortez,Adriana; Nascimento,Paula Maria Pires do; Leite,Romulo Cerqueira; Viana,João Henrique MoreiraAbstract: The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV-1) in follicular fluid and in cumulus-oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. ...