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Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail ...

Leão, Célia; Clemente, Maria; Moura, Laura; Seyfarth, Anne Mette; Hansen, Inge M.; Hendriksen, Rene S.; Amaro, Ana

The emergence and dissemination of resistance to third- and fourth-generation cephalosporins among Enterobacteriaceae from different sources impose a global public health threat. Here, we characterized by whole-genome sequencing four Escherichia coli strains harboring the blaCTX−M−65 gene identified among 49 isolates from beef and pork collected at retail. The genomic content was determined using the Center for...


Proficiency Testing of Virus Diagnostics Based on Bioinformatics Analysis of Si...

Brinkmann, Annika; Andrusch, Andreas; Belka, Ariane; Wylezich, Claudia; Höper, Dirk; Pohlmann, Anne; Nordahl Petersen, Thomas; Lucas, Pierrick

Quality management and independent assessment of high-throughput sequencing-based virus diagnostics have not yet been established as a mandatory approach for ensuring comparable results. The sensitivity and specificity of viral high-throughput sequence data analysis are highly affected by bioinformatics processing using publicly available and custom tools and databases and thus differ widely between individuals...


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