179 documents found, page 1 of 18
COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of Eur...
Honrado, Mónica; Quaresma, Andreia; Henriques, Dora; Pinto, Maria Alice; Amaral, Joana S.Honey is a widely consumed food product frequently subjected to adulteration, with the mislabeling of its botanical or geographical origin being one of the most common practices. Determining the entomological origin of honey is particularly challenging but of high relevance for ensuring its authenticity, especially for products with protected designation of origin (PDO) labels. This study presents a novel DNA m...
Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content
Yadró, Carlos A.; Lopes, Ana Rita; Henriques, Dora; Musin, Eduard; Wegener, Jakob; Pinto, M. AliceGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing...
Development of LAMP Primers for the Detection of Pyrethroid Resistance Mutation...
Costa, Maíra; Yadró García, Carlos A.; Lopes, Ana; Bejaoui, Mohamed Khalil; Almeida, Jhennifer; Correia, Lucas; Sánchez, Sara; Li, FernandaVarroa destructor is one of the main threats to Apis mellifera L., directly affecting colony health and contributing to their global decline. Control of this mite is traditionally achieved using acaricides, with pyrethroids such as tau-fuvalinate and fumethrin being the most used, acting on voltage-gated sodium channels (VGSC). However, the intensive use of these compounds by beekeepers has led to the emergence...
Large Scale Mitochondrial Evidence of C-lineage Dominance in European Honey Bees
Li, Fernanda; Lopes, Ana; Costa, Maíra; Henriques, Dora; Quaresma, Andreia; Yadró García, Carlos A.; Albo, Alexandre; Čereškienė, Laima BlažytėIn Europe, distribution of the several endemic honey bee (Apis mellifera) subspecies has suffered a considerable shift in the last century. In particular, beekeepers tend to favour subspecies of Eastern European ancestry (C-lineage), such as the Italian honey bee (A. m. ligustica), due to their perceived docility and high honey production. As a result, large scale migratory beekeeping and trade of C-lineage que...
Bioinformatics pipeline to evaluate patterns of diversity in detoxification gen...
Barbosa, Daniela; Li, Fernanda; Bashir, Sana; Lopes, Ana; Yadró García, Carlos A.; Quaresma, Andreia; Rufino, José; Rosa-Fontana, AnneliseThe Western honey bee, Apis mellifera, displays significant genetic diversity in detoxification genes, which is pivotal for environmental adaptation and resilience. Herein, we developed a bioinformatics pipeline to investigate patterns of diversity in these genes, focusing on single nucleotide polymorphisms (SNPs) across A. mellifera populations, with variant annotation performed using both snpEff and the Varia...
Human-mediated introgression and Varroa destructor shaped the genetic structure...
Henriques, Dora; Lopes, Ana; Low, Matthew; Pinto, M. AliceThe evolutionary trajectory of island populations can be rapidly altered by human-mediated migration, a process further exacerbated when immigrants introduce invasive parasites, creating new selective pressures. Using customised SNP panels constructed with genome-wide diagnostic loci, we describe the genetic changes in honey bee populations inhabiting the Azores archipelago. As part of a breeding initiative in ...
Climate change-induced stress in the honey bee Apis mellifera L.- a genetic review
Sagastume, Soledad; Cilia, Giovanni; Henriques, Dora; Yadró García, Carlos A.; Corona, Miguel; Higes, Mariano; Pinto, M. Alice; Nanetti, AntonioClimate change is a powerful driver of stress, as it reinforces hotter and drier environments. For bees, the most concerning aspects of these new environmental conditions are the resistance and resilience of bees to changes in temperature, humidity and ultraviolet radiation, as well as the negative effect on diversity of food resources which can lead in nutritional stress. The climatic vulnerability of various ...
Note: Cytonuclear patterns of a honey bee population from the Azores show a sta...
Henriques, Dora; Lopes, Ana; Costa, Maíra; Quaresma, Andreia; Doblas-Bajo, Mónica; Pinto, M. AliceThe Azores archipelago has been the stage for multiple introductions of Apis mellifera from varying origins, which have led to widespread admixture and the existence of phenotypically and genotypically heterogeneous populations. This is evident on the São Miguel Island, where the historically introduced black phenotype of A. m. iberiensis (lineage M) co-exists with the contemporaneously introduced yellow phenot...
Genomic insights into middle eastern honey bee subspecies: population structure...
Henriques, Dora; Yadró Garcia, Carlos A.; Honrado, Mónica; Amaral, Joana S.; Muz, Mustafa Necati; Muz, Dilek; Haddad, Nizar; Al Shagour, BananThe genetic patterns of Middle Eastern A. mellifera subspecies have been understudied, hindering a comprehensive understanding of honey bee evolutionary history. Here, we studied the genetic integrity of five Middle Eastern subspecies across a broad geographical range: Turkey (A. m. anatoliaca, N=97; A. m. caucasia, N=75; A. m. syriaca, N=18), Jordan and Lebanon (A. m. syriaca, N=238 and N=29), Iran (A. m. meda...
Maternal ancestry of honey bees on São Miguel-Azores (Portugal)
Costa, Maíra; Lopes, Ana; Doblas-Bajo, Mónica; Quaresma, Andreia; Barreira, Diogo; Henriques, Dora; Pinto, M. AliceThe Apis mellifera iberiensis was introduced to the Azores in the 16th century by Portuguese settlers likely from the northern part of mainland Portugal. Many years later, in the 1980s, occurred a new introduction using honey bee subspecies from C-lineage maternal ancestry. This left a C-lineage signature in the Azores, with some islands, such as Pico or Graciosa, showing high frequency of C-lineage maternal an...