4 documents found, page 1 of 1
Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
Saldanha, Carlota; Freitas, Teresa; Herdade, Ana Santos SilvaTimolol inhibits erythrocyte membrane acetylcholinesterase (AChE) enzyme activity originates less active enzyme complex forms unable to change the normal values of the nitric oxide (NO) efflux from erythrocytes. The active complex resulting from acetylcholine (ACh) binding to AChE induces higher values of NO efflux. Increased values of NO efflux were also observed in presence of high fibrinogen (Fib) concentrat...
Hydrodynamics of a free-flowing leukocyte toward the endothelial wall
Herdade, Ana Santos Silva; Sequeira, Adélia; Calado, Ângelo; Saldanha, Carlota; Kafi, OualidLeukocyte recruitment is an essential stage of the inflammatory response and although the molecular mechanisms of this process are relatively well known, the influence of the hydrodynamic effects that govern the inflammatory response are still under study. In this paper we made use of the images and experimental parameters obtained by intravital microscopy in an in vivo animal model of inflammation to track the...
Erythrocyte deformability : a partner of the inflammatory response
Herdade, Ana Santos Silva; Andolina, Giulia; Faggio, Caterina; Calado, Ângelo; Saldanha, CarlotaWe aim to establish an in vivo animal model of acute inflammation using PAF (platelet activating factor) as inflammatory agent and to study the erythrocyte deformability changes induced by the inflammatory response. Counting the number of rolling and adherent neutrophils to endothelium after 2, 4 and 6 h of intrascrotal injection of PAF we showed the induction of an inflammatory state. Blood samples are collect...
Application of a nitric oxide sensor in biomedicine
Saldanha, Carlota; Almeida, José Pedro Lopes de; Herdade, Ana Santos SilvaIn the present study, we describe the biochemical properties and effects of nitric oxide (NO) in intact and dysfunctional arterial and venous endothelium. Application of the NO electrochemical sensor in vivo and in vitro in erythrocytes of healthy subjects and patients with vascular disease are reviewed. The electrochemical NO sensor device applied to human umbilical venous endothelial cells (HUVECs) and the de...