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Exploring tomato phenotypic variability under combined nitrogen and water deficit

Machado, Joana; Heuvelink, Ep; Vasconcelos, Marta W.; Cunha, Luís M.; Finkers, Richard; Carvalho, Susana M. P.

Background: Despite drought and nitrogen (N) deficit being two of the most important crops’ growth limiting factors, only few studies have explored tomato phenotypic variability in response to both abiotic stresses. Aims: In this study, we aimed to perform a phenotypic evaluation and an analysis of the growth traits of 40 tomato genotypes (mostly focusing on old cultivars, but also including modern hybrids and ...


The effect of silicon on the antioxidant system of tomato seedlings exposed to ...

Machado, Joana; Fernandes, Ana Patricia G.; Bokor, Boris; Vaculík, Marek; Heuvelink, Ep; Carvalho, Susana M. P.; Vasconcelos, Marta W.

Exploring sustainable strategies for improving crop water and nitrogen use efficiency is essential. Silicon (Si) has been reported as a beneficial metalloid for plants since it alleviates several abiotic stresses (including drought) by triggering the plants´ antioxidant system. However, its role in mitigating the negative impact of nitrogen (N) deficit alone or when combined with water (W) deficit is not well s...


Enzymatic and non-enzymatic antioxidant responses of young tomato plants (cv. m...

Machado, Joana; Vasconcelos, Marta W.; Soares, Cristiano; Fidalgo, Fernanda; Heuvelink, Ep; Carvalho, Susana M. P.

This study aims to perform a broad analysis of the antioxidant (AOX) responses of young tomato plants exposed to single and combined mild nitrogen (N) and water deficits through the evaluation of oxidative biomarkers, non-enzymatic and enzymatic AOX components. ‘Micro-Tom’ seedlings were subjected to four treatments: control (CTR; 100%N + 100%W), N deficit (N; 50%N), water deficit (W; 50%W), and combined defici...


Silicon improves tomato plants’ performance under individual and combined nitro...

Machado, Joana; Fernandes, Ana P.G.; Heuvelink, Ep; Carvalho, Susana M.P.; Vasconcelos, Marta W.


Young tomato plants respond differently under single or combined mild nitrogen ...

Machado, Joana; Vasconcelos, Marta W.; Soares, Cristiano; Fidalgo, Fernanda; Heuvelink, Ep; Carvalho, Susana M.P.

This study aimed to understand the morphophysiological responses and primary metabolism of tomato seedlings subjected to mild levels of nitrogen and/or water deficit (50% N and/or 50% W). After 16 days of exposure, plants grown under the combined deficit showed similar behavior to the one found upon exposure to single N deficit. Both N deficit treatments resulted in a significantly lower dry weight, leaf area, ...


Moderate salinity improves stomatal functioning in rose plants grown at high re...

Carvalho, Dália R. A.; Vasconcelos, Marta W.; Lee, Sangseok; Vreugdenhil, Dick; Heuvelink, Ep; Carvalho, Susana M. P.

Plants grown at high relative air humidity (RH ≥ 85%) show hampered stomatal closure in response to closing stimuli. We hypothesized that a moderate salinity during growth could trigger a stress response and stimulate stomatal functioning due to an increased leaf abscisic acid concentration ([ABA]). Cut rose ‘Prophyta’ was grown at moderate (63%) or high (89%) RH combined with three electrical conductivities (E...


Root-to-shoot ABA signaling does not contribute to genotypic variation in stoma...

Carvalho, Dália R. A.; Fanourakis, Dimitrios; Correia, Maria J.; Monteiro, José A.; Araújo-Alves, José Pedro L.; Vasconcelos, Marta W.

High relative air humidity (RH 85%) during leaf expansion hampers stomatal responsiveness to closing stimuli, a genotype-dependent effect. Genotypes with reduced stomatal closure in response to closing stimuli (i.e., sensitive genotypes) show low bulk leaf abscisic acid concentration ([ABA]). We hypothesized that the amount of ABA produced in the roots and delivered with the transpiration stream to the leaves i...


Gene expression and physiological responses associated to stomatal functioning ...

Carvalho, Dália R.A.; Vasconcelos, Marta W.; Lee, Sangseok; Koning-Boucoiran, Carole F.S.; Vreugdenhil, Dick; Krens, Frans A.; Heuvelink, Ep

tHigh relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessivewater loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid(ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA relatedgenes (involved in the water stress response) aiming to better understand the mechanisms underlyingcon...


QTL analysis for stomatal functioning in tetraploid Rosa × hybrida grown at hig...

Carvalho, Dália R. A.; Koning-Boucoiran, Carole F. S.; Fanourakis, Dimitrios; Vasconcelos, Marta W.; Carvalho, Susana M. P.; Heuvelink, Ep

High relative air humidity (RH >= 85 %) during leaf development disturbs stomatal functioning leading to excessive water loss in conditions of high evaporative demand, resulting in severe reduction in postharvest longevity. In roses, this effect depends on the genotype, opening the possibility for breeding cultivars with more responsive stomata. In this study, we aim at identifying genomic regions associated wi...


Elevated air movement enhances stomatal sensitivity to abscisic acid in leaves ...

Carvalho, Dália R. A.; Torre, Sissel; Kraniotis, Dimitrios; Almeida, Domingos P. F.; Heuvelink, Ep; Carvalho, Susana M. P.

High relative air humidity (RH ≥ 85%) during growth leads to stomata malfunctioning, resulting in water stress when plants are transferred to conditions of high evaporative demand. In this study, we hypothesized that an elevated air movement (MOV) 24 h per day, during the whole period of leaf development would increase abscisic acid concentration ([ABA]) enhancing stomatal functioning. Pot rose 'Toril' was grow...


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