2 documents found, page 1 of 1
MHC Variants Associated With Symptomatic Versus Asymptomatic SARS-CoV-2 Infecti...
Castelli, Erick C. [UNESP]; de Castro, Mateus V.; Naslavsky, Michel S.; Scliar, Marilia O.; Silva, Nayane S. B. [UNESP]; Andrade, Heloisa S. [UNESP]Made available in DSpace on 2022-04-29T08:46:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-28; Center for Information Technology; Center for Scientific Review; National Institutes of Health; Office of Extramural Research, National Institutes of Health; Office of Research Infrastructure Programs, National Institutes of Health; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fu...
Application of BRED technology to construct recombinant D29 reporter phage expr...
Silva, Joas L. da; Piuri, Mariana; Broussard, Gregory; Marinelli, Laura J.; Bastos, Gisele M.; Hirata, Rosario D.C.; Hatfull, Graham F.Bacteriophage Recombineering of Electroporated DNA (BRED) has been described for construction of gene deletion and point mutations in mycobacteriophages. Using BRED, we inserted a Phsp60-egfp cassette (1143 bp) into the mycobacteriophage D29 genome to construct a new reporter phage, which was used for detection of mycobacterial cells. The cassette was successfully inserted and recombinant mycobacteriophage puri...