23 documents found, page 1 of 3

Uso de jogos didáticos na aprendizagem de biologia celular: estudo antes e depo...

Considerando a importância da utilização de métodos ativos para facilitar o processo educacional, o trabalho analisou a efetividade do uso de jogos didáticos como meio de aproximação do aluno ao conteúdo teórico. A aplicação dos jogos foi realizada com estudantes do primeiro semestre dos cursos de Ciências Biológicas e de Biotecnologia da Universidade Federal do Ceará (UFC). Os alunos jogaram antes e depois da ...

Protein’s race e o jogo dos lissosomos: um relato sobre a aplicação de jogos di...

Por se tratarem de temas complexos e dinâmicos, os temas envolvendo a Biologia Celular se tornam de difícil assimilação. Os jogos didáticos podem ser utilizados como uma ferramenta para contrapor essa dificuldade, pois utilizam o lúdico e o sentimento de prazer como facilitador da aprendizagem. Portanto, pensou-se na elaboração e aplicação de dois jogos didáticos a fim de auxiliar a aprendizagem dos assuntos de...

Proteomics analysis of zebrafish larvae exposed to 3,4-dichloroaniline using th...

The zebrafish (Danio rerio) is a small teleost fish that is becoming increasingly popular in laboratories worldwide and several attributes have also placed the zebrafish under the spotlight of (eco)toxicological studies. Since the 1990s, international organizations such as ISO and OECD have published guidelines for the use of zebrafish in ecotoxicological assessment of environmental toxicants such as the Fish E...

Proteína surfactante de Leptodactylus Vastus A. Lutz 1930: do seu local de prod...

Assessing the effects of an acute exposure to worst-case concentration of Cry p...

Cry1C, Cry1F e Cry1Ab são proteínas inseticidas de Bacillus thuringiensis(Bt) que são expressos em culturas transgênicas. Dada a entrada dessas proteínas em ambientes aquáticos, é relevante avaliarseus impactos nos organismos aquáticos. Neste trabalho, buscamos avaliar os efeitos de Cry1C, Cry1F eCry1Ab na zebrafish embriões e larvas de uma concentração prevista de pior cenário destes proteínas (definido para 1...

Alternative Heterologous Expression of l‑Arabinose Isomerase from Enterococcus ...

This study reports an alternative strategy for the expression of a recombinant l-AI from Enterococcus faecium DBFIQ E36 by auto-induction using glucose and glycerol as carbon sources and residual whey lactose as inducer agent. Commercial lactose and isopropyl β-d-1-thiogalactopyranoside (IPTG) were also evaluated as inducers for comparison of enzyme expression levels. The enzymatic extracts were purifed by afni...

A novel bacterial carboxylesterase identified in a metagenome derived‑clone fro...

A functional screening of 1152 clones from a plasmid library constructed with DNA extracted from Brazilian mangrove sediments revealed 3 positive clones for ester-hydrolyzing enzymes, or about one lipolytic gene per 1.2 Mb DNA, which corroborates the idea that oil-contaminated mangroves are a good source of novel microbial lipases/esterases. The partial sequence of the clone LipG7 (1179 bp) showed 30.2% of pred...

Biochemical Characterization of Heat-Tolerant Recombinant lArabinose Isomerase ...

d-Tagatose is a ketohexose, which presents unique properties as a low-calorie functional sweetener possessing a sweet flavor profile similar to d-sucrose and having no aftertaste. Considered a generally recognized as safe (GRAS) substance by FAO/ WHO, d-tagatose can be used as an intermediate for the synthesis of other optically active compounds as well as an additive in detergent, cosmetic, and pharmaceutical ...

First insights into insecticidal activity against Aedes aegypti and partial bio...

BACKGROUND: Arboviroses such as dengue, Zika and chikungunya represent a serious public health issue as a consequenceof the absence of approved vaccines or specific antiviral drugs against the arboviruses that cause them. One way to preventthese diseases is by combating the vector mosquito, Aedes aegypti (Diptera), which has serine proteases in the midgut. Proteaseinhibitors are molecules that can block enzyme a...

One-step immobilization and stabilization of a recombinant Enterococcus faecium...

A recombinant L-arabinose isomerase from Enterococcus faecium DBFIQ E36 was immobilized onto multifunctional epoxide supports by chemical adsorption and onto a chelate-activated support via polyhistidine-tag, located on the N-terminal (N-His-L-AI) or on the C-terminal (C-His-L-AI) sequence, followed by covalent bonding between the enzyme and the support. The results were compared to reversible L-AI immobilizati...