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Gap-analysis and annotated reference library for supporting macroinvertebrate m...

Leite, B. R.; Vieira, Pedro Emanuel Ferreira Reis; Teixeira, M. A. L.; Lobo-Arteaga, J.; Hollatz, C.; Borges, L. M. S.; Duarte, Sofia Alexandra Ferreira

DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy of species-level assignment, and consequent taxonomic coverage, relies on comprehensive DNA barcode reference libraries, which, due to incompleteness, are currently a recognized limitation for metabarcoding applications. In this study, we assembled a comprehensive reference library of DNA barcodes for...


With a little help from DNA barcoding: investigating the diversity of Gastropod...

Borges, L. M. S.; Hollatz, C.; Lobo, J.; Cunha, Ana; Vilela, A. P.; Calado, G.; Coelho, R.; Costa, A. C.; Ferreira, M. G.; Costa, M. H.

The Gastropoda is one of the best studied classes of marine invertebrates. Yet, most species have been delimited based on morphology only. The application of DNA barcodes has shown to be greatly useful to help delimiting species. Therefore, sequences of the cytochrome c oxidase I gene from 108 specimens of 34 morpho-species were used to investigate the molecular diversity within the gastropods from the Portugue...


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