8 documents found, page 1 of 1
Six new Phytophthora species from ITS Clade 7a including two sexually functiona...
Jung, Thomas; Horta, Marília; Scanu, B.; Seress, D.; Kovacs, G. M.; Maia, Cristiana; Perez-Sierra, A.; Chang, T. -T.; Chandelier, A.; Heungens, K.During a survey of Phytophthora diversity in natural ecosystems in Taiwan six new species were detected. Multigene phylogeny based on the nuclear ITS, beta-tubulin and HSP90 and the mitochondrial cox1 and NADH1 gene sequences demonstrated that they belong to ITS Clade 7a with P. europaea, P. uniformis, P. rubi and P. cambivora being their closest relatives. All six new species differed from each other and from ...
A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...
Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria; Almeida, Paulo; Almeida, Tânia; Amorim, Maria; Araújo, SusanaCork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork ...
A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...
Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria Helena; Almeida, Paulo; Almeida, Tânia; Amorim, Maria Isabel; Araújo, SusanaBackground: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...
A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...
Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, SusanaBackground: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...
Quercus suber – P. cinnamomi interaction: hypothetical molecular mechanism model
Coelho, A. C.; Horta, Marília; Ebadzad, G.; Cravador, A.Phytophthora cinnamomi Rands is involved in the decline and mortality of Quercus suber L. and Quercus ilex L. in Southern Europe, in particular in Portugal and Spain. The presence and spread of P. cinnamomi in these regions is a severe threat to these oak ecosystems leading to expectable severe consequences for the production of cork and acorns in the near future. Molecular mechanisms underlying oomycete-host i...
Involvement of the beta-cinnamomin elicitin in infection and colonisation of co...
Cravador, A.; Horta, Marília; Caetano, P.; Medeira, C.; Maia, I.The virulence of two wild type (PA45 and PA37) and two genetically modified (13C: hygromycin resistant; FATSS: hygromycin resistant and β-cin knock-down) Phytophthora cinnamomi strains towards cork oak (Quercus suber) was assessed via a quantitative evaluation of disease symptoms arising from a soil infestation assay, and by a istological analysis of root colonization. Comparison of virulence, as expressed by s...
In vitro and in vivo quantification of elicitin expression in Phytophthora cinn...
Horta, Marília; Sousa, Nelson; Coelho, A. C.; Neves, D.; Cravador, A.The differential expression of four Phytophthora cinnamomi elicitin genes was analysed by Real Time RT-PCR. In in vitro cultures, the a-cinnamomin gene showed the highest level of expression, the b-cinnamomin gene (b-cin) was the most inducible, and the HAE transcripts were in low abundance. Transcription of all the elicitins was active during the active growth of the pathogen when infecting cork oak (Quercus s...
Involvement of a cinnamyl alcohol dehydrogenase of Quercus suber in the defence...
Coelho, A. C.; Horta, Marília; Neves, D.; Cravador, A.A gene encoding a potential NADPH-dependent cinnamyl alcohol dehydrogenase (QsCAD1) (GenBank accession no: AY362455) was identified in Quercus suber (cork oak). Its complete cDNA sequence was obtained by RACE-PCR, starting from total RNA extracted from roots of seedlings of Q. suber, infected with Phytophthora cinnamomi, the causal agent of the decline and sudden death of Q. suber and Quercus ilex subsp. rotund...