24 documents found, page 1 of 3

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High variability in aggression and habituation to the mirror assay in ornamenta...

Silva, Melina Coelho da; Canário, Adelino V. M.; Hubbard, Peter C.; Cardoso, Sara D.; Gonçalves, David; da Silveira Gonçalves, David Miguel

Understanding consistent inter-individual variability in animal behaviour, known as personality traits, is essential for exploring the mechanisms and evolutionary consequences of behavioural diversity. Aggressive behaviour influences survival, resource acquisition, and reproduction, so clarifying individual differences can enhance our understanding of ecological dynamics and improve experimental design accuracy...


Ultra-low noise PEDOT:PSS electrodes on bacterial cellulose: a sensor to access...

Inácio, Pedro M.C.; Medeiros, Maria C.R.; Carvalho, Tiago; Félix, Rute C.; Mestre, Ana; Hubbard, Peter C.; Ferreira, Quirina; Morgado, Jorge

This study is focused on the particular advantages of organic-based devices to measure cells that do not generate action potentials, also known as non-electrogenic cells. While there is a vast literature about the application of organic conductors to measure neurons, cardiomyocytes and brain tissues, electrical measurements of non-electrogenic cells are rare. This is because non-electrogenic cells generate weak...


An electrical method to measure low-frequency collective and synchronized cell ...

Medeiros, Maria C. R.; Mestre, Ana; Inácio, Pedro; Asgarif, Sanaz; Araújo, Inês M.; Hubbard, Peter C.; Velez, Zélia; Cancela, M. Leonor

An electrical method to measure extracellular bioelectrical activity in vitro is presented. This method exploits the Helmholtz capacitive double-layer established at the electrode surface. Small extracellular voltage variations in the order of μVs induce through the double-layer capacitor a displacement current that is measured. This current is then enhanced by a gain factor proportional to the electrode capaci...


A multi-component pheromone in the urine of dominant male tilapia (Oreochromis ...

Keller-Costa, Tina; Saraiva, João L.; Hubbard, Peter C.; Barata, Eduardo N.; Canário, Adelino V. M.

Males often use scent to communicate their domi- nance, and to mediate aggressive and breeding behaviors. In teleost fish, however, the chemical composition of male pher- omones is poorly understood. Male Mozambique tilapia, Oreochromis mossambicus, use urine that signals social status and primes females to spawn. The urinary sex pheromone di- rected at females consists of 5β-pregnane-3α,17α,20β-triol 3- glucur...


Identity of a Tilapia Pheromone Released by Dominant Males that Primes Females ...

Keller-Costa, Tina; Hubbard, Peter C.; Paetz, Christian; Nakamura, Yoko; da Silva, José P.; Rato, A.E.; Barata, Eduardo N.; Schneider, Bernd

Knowledge of the chemical identity and role of urinary pheromones in fish is scarce, yet it is necessary in order to understand the integration of multiple senses in adaptive responses and the evolution of chemical communication [1]. In nature, Mozambique tilapia (Oreochromis mossambicus) males form hierarchies, and females mate preferentially with dominant territorial males, which they visit in aggregations or...


Olfactory transduction pathways in the Senegalese sole Solea senegalensis

Velez, Zélia; Hubbard, Peter C.; Barata, Eduardo N.; Canário, Adelino V.M.

This study tested whether differences in sensitivity between the upper and lower olfactory epithelia of Solea senegalensis are associated with different odorant receptors and transduction pathways, using the electro-olfactogram. Receptor mechanisms were assessed by cross-adaptation with amino acids (L-cysteine, L-phenylalanine and 1-methyl-L-tryptophan) and bile acids (taurocholic acid and cholic acid). This su...


Muscular hypertrophy of urinary bladders in dominant tilapia facilitates the co...

Keller-Costa, Tina; Lopes, Orlando; Almeida, Olinda; Hubbard, Peter C.; Iacovella, Adele; Lima, Monica; Barata, Eduardo N.; Canário, Adelino V.M.

The urination pattern of the Mozambique tilapia (Oreochromis mossambicus) depends on social context, and the olfactory potency of urine released depends on social rank (males) and reproductive status (females). This strongly suggests that urine mediates chemical communication in this species. The current study tested, firstly, whether urine production rate depends on sex or social status and, secondly, whether d...


Olfactory sensitivity to amino acids in the blackspot sea bream (Pagellus bogar...

Hubbard, Peter C.; Barata, Eduardo N.; Ozório, Rodrigo O. A.; Valente, Luisa M. P.; Canário, Adelino V. M.

The current study investigated the olfactory sensitivity of the blackspot sea bream to amino acids, odorants associated with food detection in fsh, and compared the efficacy of two different experimental methods: multiunit recording from the olfactory nerve and the electro-olfactogram (EOG). Twenty essential amino acids plus L-DOPA evoked clear, concentration-dependent olfactory responses using both methods, wi...


Olfactory Sensitivity to Amino Acids in the Blackspot Seabream (Pagellus Bogara...

Barata, Eduardo N.; Ozorio, Rodrigo O.A.; Hubbard, Peter C.; Valente, Luisa M.P.; Canário, Adelino V.M.

Intensive aquaculture is heavily dependent on high-quality fishmeal to meet their protein requirements. However, due to the increased demand, decreased availability, raising costs and the potential presence of organic and inorganic contaminants, it is of economical and environmental interest to replace fishmeal by a renewable protein sources. This is particularly challenging since replacing fishmeal may lack ap...


Seasonal cell differentiation and olfactory potency of secretions by the anal g...

Barata, Eduardo N.; Lopes, Orlando; Araújo, Jorge; Serrano, Rui M.; Hubbard, Peter C.; Canário, Adelino V.M.

The current study shows cytological differences between the anal glands of pre-spawning and spawning peacock blennies Salaria pavo. The cytological differences in the anal gland coincide with higher olfactory potency of their secretions in spawning males, suggesting that seasonal differentiation of cell clusters secreting neutral mucins is related to the production and release of the putative sex pheromone.


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