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Almeida, Micael; Inácio, José M.; Vital, Carlos M.; Rodrigues, Madalena R.; Araújo, Beatriz C.; Belo, José A.

After the publication of the review article [1], one of the referenced manuscripts was found to have been retracted. To enhance the quality of the manuscript, we decided to replace the previous reference number 157 [2] from the original manuscript with the new reference [3] at the same position in the reference list. Original reference number 157: Zhang, D.; Ning, J.; Ramprasath, T.; Yu, C.; Zheng, X.; Song, P....


Generation and characterization of a human iPS cell line from a patient-related...

Pars, Selin; Cristo, Fernando; Inácio, José M.; Rosas, Graça; Carreira, Isabel Marques; Melo, Joana Barbosa; Mendes, Patrícia; Martins, Duarte Saraiva

A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited...


Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation i...

Bover, Oriol; Justo, Tiago; Pereira, Paulo N. G.; Facucho-Oliveira, João; Inácio, José M.; Ramalho, José S.; Domian, Ibrahim J.; Belo, José A.

Understanding the molecular pathways regulating cardiogenesis is crucial for the early diagnosis of heart diseases and improvement of cardiovascular disease. During normal mammalian cardiac development, collagen and calcium-binding EGF domain-1 (Ccbe1) is expressed in the first and second heart field progenitors as well as in the proepicardium, but its role in early cardiac commitment remains unknown. Here we d...


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