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A chromosome-level genome resource for studying virulence mechanisms and evolut...

Peri, Tobias; Edwards, Richard; Surana, Priyanka; Mangelson, Hayley; Inácio, Vera; Silva, Maria do Céu; Guerra-Guimarães, Leonor; Park, Obert


SNP Detection in Pinus pinaster Transcriptome and Association with Resistance t...

Modesto, Inês; Inácio, Vera; Novikova, Polina; Carrasquinho, Isabel; Van de Peer, Yves; Miguel, Célia M.

Pinewood nematode (PWN, Bursaphelenchus xylophilus) is the causal agent of pine wilt disease (PWD), which severely affects Pinus pinaster stands in southwestern Europe. Despite the high susceptibility of P. pinaster, individuals of selected half-sib families have shown genetic variability in survival after PWN inoculation, indicating that breeding for resistance can be a valuable strategy to control PWD. In thi...


A chromosome-level genome resource for studying virulence mechanisms and evolut...

Tobias, Peri A; Edwards, Richard J.; Surana, Priyanka; Mangelson, Hayley; Inácio, Vera; Silva, Maria do Céu; Varzea, Vitor; Park, Robert F.

Recurrent epidemics of coffee leaf rust, caused by the fungal pathogen Hemileia vastatrix, have constrained the sustainable production of Arabica coffee for over 150 years. The ability of H. vastatrix to overcome resistance in coffee cultivars and evolve new races is inexplicable for a pathogen that supposedly only utilizes clonal reproduction. Understanding the evolutionary complexity between H. vastatrix and ...


Cork cells in cork oak periderms undergo programmed cell death and proanthocyan...

Inácio, Vera; Lobato, Carolina; Graça, José; Morais-Cecilio, Leonor

Vascular plants with secondary growth develop a periderm mostly composed of dead suberized cork cells to face environmental hostile conditions. Cork oak has a highly active and long-living phellogen forming a remarkably thick periderm that is periodically debarked for industrial purposes. This wounding originates the quick formation of a new traumatic periderm, making cork oak an exceptional model to study the ...


ChlP-Seq reveals that QsMYB1 directly targets genes involved in lignin and sube...

Capote, Tiago; Barbosa, Pedro; Usié, Ana; Ramos, António Marcos; Inácio, Vera; Ordás, Ricardo; Gonçalves, Sónia; Morais-Cecilio, Leonor

Background: Gene activity is largely controlled by transcriptional regulation through the action of transcription factors and other regulators. QsMYB1 is a member of the R2R3-MYB transcription factor family related to secondary growth, and in particular, with the cork development process. In order to identify the putative gene targets of QsMYB1 across the cork oak genome we developed a ChIP-Seq strategy. Result...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria; Almeida, Paulo; Almeida, Tânia; Amorim, Maria; Araújo, Susana

Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork ...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria Helena; Almeida, Paulo; Almeida, Tânia; Amorim, Maria Isabel; Araújo, Susana

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, Susana

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...


Genomic organization and dynamics of repetitive DNA sequences in representative...

Alves, Sofia; Ribeiro, Teresa; Inácio, Vera; Rocheta, Margarida; Morais-Cecílio, Leonor

Oaks, chestnuts, and beeches are economically important species of the Fagaceae. To understand the relationship between these members of this family, a deep knowledge of their genome composition and organization is needed. In this work, we have isolated and characterized several AFLP fragments obtained from Quercus rotundifolia Lam. through homology searches in available databases. Genomic polymorphisms involvi...


Improving the enantioselectivity of Bacillus subtilis LipA variants

Inácio, Vera

This thesis describes the application of lipase A (lipA) from Bacillus subtilis 168 which is of particular importance for the production of enantiomer of interest S-(+)- 1, 2- O – isopropylidene-sn-glycerol (IPG) out of its racemic ester, as it shows some advantageous characteristics compared to other lipases. In directed evolution experiments, success strongly depends on the availability of screening or select...


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