10 documents found, page 1 of 1
Probe-based metagenomic pathogen detection
Ferreira, Rita; Coelho, Luís; Santos, João Dourado; Sobral, Daniel; Isidro, Joana; Mixão, Verónica; Pinto, Miguel; Nunes, Alexandra; Borrego, Maria JoséPublisher Copyright: Copyright © 2025 Ferreira, Coelho, Santos, Sobral, Isidro, Mixão, Pinto, Nunes, Borrego, Lopo, Oleastro, Sousa, Palminha, Veríssimo, Gargaté, Guiomar, Cordeiro, Macedo, Bajanca-Lavado, Paixão, Duarte, Vieira, Borges and Gomes.; Probe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small ...
Comparative complete scheme and booster effectiveness of COVID-19 vaccines in p...
Kislaya, Irina; Peralta-Santos, André; Borges, Vítor; Vieira, Luís; Sousa, Carlos; Ferreira, Bibiana; Pelerito, Ana; Gomes, João PauloFunding Information: The acquisition of sequencing equipment and reagents used in this study by the Instituto Nacional de Saúde Doutor Ricardo Jorge was partially funded by the HERA project (grant no. 2021/PHF/23776), supported by the European Commission through the European Centre for Disease Control, and also partially funded by the Genome PT project (grant no. POCI‐01‐0145‐FEDER‐022184), supported by COMPETE...
Mutation rate of SARS-CoV-2 and emergence of mutators during experimental evolu...
Amicone, Massimo; Borges, Vítor; Alves, Maria João; Isidro, Joana; Zé-Zé, Líbia; Duarte, Sílvia; Vieira, Luís; Guiomar, Raquel; Gomes, João PauloFunding: M.A. was supported by ‘Fundação para a Ciência e Tecnologia’ (FCT), fellowships PD/BD/138735/2018. Research was supported by FCT Project PTDC/BIA-EVL/31528/2017 to I.G. and by funds from Portuguese NIH.; Background and objectives: To understand how organisms evolve, it is fundamental to study how mutations emerge and establish. Here, we estimated the rate of mutation accumulation of SARS-CoV-2 in vitro...
Unraveling the hurdles of a large Covid-19 epidemiological investigation by vir...
Sá, Regina; Isidro, Joana; Borges, Vítor; Duarte, Sílvia; Vieira, Luís; Gomes, João P; Tedim, Sofia; Matias, Judite; Leite, AndreiaCopyright © 2022 The British Infection Association. Published by Elsevier Ltd. All rights reserved.; COVID-19 local outbreak response relies on subjective information to reconstruct transmission chains. We assessed the concordance between epidemiologically linked cases and viral genetic profiles, in the Baixo Vouga Region (Portugal), from March to June 2020. A total of 1925 COVID-19 cases were identified, with ...
Genetic Characterization of Brucella spp.
Pelerito, Ana; Nunes, Alexandra; Grilo, Teresa; Isidro, Joana; Silva, Catarina; Ferreira, Ana Cristina; Valdezate, Sylvia; Núncio, Maria SofiaFunding Information: Funding: This work is a result of the GenomePT project (POCI-01- 0145-FEDER-022184), supported by the COMPETE 2020 – Operational Program for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Program (Lisboa2020), Algarve Portugal Regional Operational Program (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional ...
Neisseria gonorrhoeae clustering to reveal major European whole-genome-sequenci...
Pinto, Miguel; Borges, Vítor; Isidro, Joana; Rodrigues, João Carlos; Vieira, Luís; Borrego, Maria José; Gomes, João PauloFunding information M.P. was supported by the Portuguese Science and Technology Foundation (FCT) through grant SFRH/BD/109264/2015. This work was partially funded by the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 – Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operatio...
Nosocomial Outbreak of SARS-CoV-2 in a "Non-COVID-19" Hospital Ward
Borges, Vítor; Isidro, Joana; Macedo, Filipe; Neves, José; Silva, Luís; Paiva, Mário; Barata, José; Catarino, Judite; Ciobanu, Liliana; Duarte, SílviaFunding: This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. This work is also a result of the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Progr...
Multidrug-Resistant Salmonella enterica Serovar Rissen Clusters Detected in Azo...
Silveira, Leonor; Pinto, Miguel; Isidro, Joana; Pista, Ângela; Themudo, Patrícia; Vieira, Luís; Machado, Jorge; Gomes, João PauloGastrointestinal infections caused by nontyphoidal Salmonella (NTS) remain one of the main causes of foodborne illness worldwide. Within the multiple existing Salmonella enterica serovars, the serovar Rissen is rarely reported, particularly as a cause of human salmonellosis. Between 2015 and 2017, the Portuguese National Reference Laboratory of Gastrointestinal Infections observed an increase in the number of c...
Imipenem Resistance in Clostridium difficile Ribotype 017, Portugal
Isidro, Joana; Santos, Andrea; Nunes, Alexandra; Borges, Vitor; Silva, Catarina; Vieira, Luis; Mendes, Aristides L.; Serrano, MonicaWe describe imipenem-resistant and imipenem-susceptible clinical isolates of Clostridium difficile ribotype 017 in Portugal. All ribotype 017 isolates carried an extra penicillin-binding protein gene, pbp5, and the imipenem-resistant isolates had additional substitutions near the transpeptidase active sites of pbp1 and pbp3. These clones could disseminate and contribute to imipenem resistance.
Genomic Study of a Clostridium difficile Multidrug Resistant Outbreak-Related C...
Isidro, Joana; Menezes, Juliana; Serrano, Monica; Borges, Vitor; Paixao, Pedro; Mimoso, Margarida; Martins, Filomena; Toscano, Cristina; Santos, Andrea