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Antibiotic resistance in wastewater treatment plants: Tackling the black box

Manaia, CM; Rocha, J; Scaccia, N; Marano, R; Radu, E; Biancullo, F; Cerqueira, F; Fortunato, G; Iakovides, IC; Zammit, I; Kampouris, I

Wastewater is among the most important reservoirs of antibiotic resistance in urban environments. The abundance of carbon sources and other nutrients, a variety of possible electron acceptors such as oxygen or nitrate, the presence of particles onto which bacteria can adsorb, or a fairly stable pH and temperature are examples of conditions favouring the remarkable diversity of microorganisms in this peculiar ha...


A rationale for the high limits of quantification of antibiotic resistance gene...

Fortunato, G.; Ivone Vaz Moreira; Becerra-Castro, C.; Olga C Nunes; Manaia, C.M.


Oryzisolibacter propanilivorax gen. nov., sp nov., a propanil-degrading bacterium

Ivone Vaz Moreira; Narciso da Rocha, C; Lopes, AR; Carvalho, G; Lobo da Cunha, A; Whitman, WB; Snauwaert, C; Vandamme, P; Manaia, CM; Olga C Nunes

Strain EPL6(T), a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6(T) was observed to be a member of the family Comamonadaceae, sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601(T) (96.8 %) and Melaminivora ...


Ubiquitous and persistent Proteobacteria and other Gram-negative bacteria in dr...

Ivone Vaz Moreira; Olga C. Nunes; Célia M. Manaia

Drinking water comprises a complex microbiota, in part shaped by the disinfection and distribution systems. Gram-negative bacteria, mainly members of the phylum Proteobacteria, represent the most frequent bacteria in drinking water, and their ubiquity and physiological versatility raises questions about possible implications in human health. The first step to address this concern is the identification and chara...


Multidrug Resistance in Quinolone-Resistant Gram-Negative Bacteria Isolated fro...

Ivone Vaz Moreira; Ana Rita Varela; Thamiris V. Pereira; Romário C. Fochat; Célia M. Manaia

This study is aimed to assess if hospital effluents represent an important supplier of multidrug-resistant (MDR) Gram-negative bacteria that, being discharged in the municipal collector, may be disseminated in the environment and bypassed in water quality control systems. From a set of 101 non-Escherichia coli Gram-negative bacteria with reduced susceptibility to quinolones, was selected a group of isolates com...


Draft genome sequences of two Ralstonia pickettii strains with different aminog...

Ivone Vaz Moreira; Javier Tamames; José Luis Martínez; Célia M. Manaia

The genomes of two Ralstonia pickettii strains (H2Cu2 and H2Cu5), isolated from hospital effluent in a selective medium containing CuSO4, were sequenced. They presented MICs of > 256 and 6 Îg/ml for the aminoglycoside gentamicin, respectively. The 5.2-Mb draft genomes have 40 contigs for strain H2Cu and 113 for H2Cu5.


Wastewater reuse in irrigation: A microbiological perspective on implications i...

Cristina Becerra Castro; Ana Rita Lopes; Ivone Vaz Moreira; Elisabete F. Silva; Célia M. Manaia; Olga C. Nunes

The reuse of treated wastewater, in particular for irrigation, is an increasingly common practice, encouraged by governments and official entities worldwide. Irrigation with wastewater may have implications at two different levels: alter the physicochemical and microbiological properties of the soil and/or introduce and contribute to the accumulation of chemical and biological contaminants in soil. The first ma...


Development of an automatic identification algorithm for antibiogram analysis

Luan F. R. Costa; Eduardo S. da Silva; Victor T. Noronha; Ivone Vaz Moreira; Olga C. Nunes; Marcelino M. de Andrade

Routinely, diagnostic and microbiology laboratories perform antibiogram analysis which can present some difficulties leading to misreadings and intra and inter-reader deviations. An Automatic Identification Algorithm (AIA) has been proposed as a solution to overcome some issues associated with the disc diffusion method, which is the main goal of this work. ALA allows automatic scanning of inhibition zones obtai...


Assessment of copper and zinc salts as selectors of antibiotic resistance in Gr...

Cristina Becerra Castro; Rita A. Machado; Ivone Vaz Moreira; Célia M. Manaia

Some metals are nowadays considered environmental pollutants. Although some, like Cu and Zn, are essential for microorganisms, at high concentrations they can be toxic or exert selective pressures on bacteria. This study aimed to assess the potential of Cu or Zn as selectors of specific bacterial populations thriving in wastewater. Populations of Escherichia coli recovered on metal-free and metal-supplemented c...


Hydromonas duriensis gen. nov., sp nov., isolated from freshwater

Ivone Vaz Moreira; Carlos Narciso da Rocha; Evie De Brandt; Peter Vandamme; A. C. Silva Ferreira; Alexandre Lobo da Cunha; Olga C. Nunes

An aerobic, Gram-stain-negative rod, designated strain A2P5(T), was isolated from the Douro river, in Porto, Portugal. Cells were catalase- and oxidase-positive. Growth occurred at 15-30 degrees C, at pH 6-8 and in the presence of 1 % (w/v) NaCl. The major respiratory quinone was Q8, the genomic DNA had a G+C content of 47 +/- 1 mol%, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol...


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