3 documents found, page 1 of 1
Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell s...
Pirraco, Rogério; Iwata, Takanori; Yoshida, T.; Marques, A.; Yamato, Masayuki; Reis, R. L.; Okano, T.Addressing the problem of vascularization is of vital importance when engineering three-dimensional (3D) tissues. Endothelial cells are increasingly used in tissue-engineered constructs to obtain prevascularization and to enhance in vivo neovascularization. Rat bone marrow stromal cells were cultured in thermoresponsive dishes under osteogenic conditions with human umbilical vein endothelial cells (HUVECs) to o...
Development of osteogenic cell sheets for bone tissue engineering applications
Pirraco, Rogério; Obokata, Haruko; Iwata, Takanori; Marques, A. P.; Tsuneda, Satoshi; Yamato, Masayuki; Reis, R. L.; Okano, T.The use of scaffolds in combination with osteogenic cells has been the gold standard in bone tissue engineering strategies. These strategies have, however, in many cases failed to produce the desired results due to issues such as the immunogenicity of the biomaterials used and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrients diffusion. Here, we originally propose the use of ce...
The potential of osteogenic cell sheets co-cultured with endothelial cells for ...
Pirraco, Rogério; Iwata, Takanori; Marques, A. P.; Yamato, Masayuki; Reis, R. L.; Okano, T.