12 documents found, page 1 of 2
Exploring Omega-3′s impact on the expression of Bone-Related Genes in Meagre (A...
Luján-Amoraga, Leticia; Delgado-Martín, Belén; Lourenço-Marques, Cátia; J. Gavaia, Paulo; Bravo, Jimena; Bandarra, Narcisa M.; Dominguez, DavidDietary supplementation with Omega-3 fatty acids seems to promote skeletal health. Therefore, their consumption at imbalanced or excessive levels has offered less beneficial or even prejudicial effects. Fish produced in aquaculture regimes are prone to develop abnormal skeletons. Although larval cultures are usually fed with diets supplemented with Omega-3 Long Chain Polyunsaturated fatty acids (LC-PUFAs), the ...
The osteogenic and mineralogenic potential of the microalgae Skeletonema costat...
Carletti, Alessio; Rosa, Joana; Pes, Katia; Borges, Inês; Santos, Tamara; Barreira, Luísa; Varela, João; Pereira, Hugo; Cancela, M. LeonorSkeletal disorders are problematic aspects for the aquaculture industry as skeletal deformities, which affect most species of farmed fish, increase production costs and affect fish welfare. Following recent findings that show the presence of osteoactive compounds in marine organisms, we evaluated the osteogenic and mineralogenic potential of commercially available microalgae strains Skeletonema costatum and Tet...
Type I Diabetes in Zebrafish reduces sperm quality and increases Insulin and Gl...
Diogo, Patricia; Martins, Gil; F. Simao, Marcio; Marreiros, Ana; Eufrásio, Ana Catarina; Cabrita, Elsa; J. Gavaia, PauloType I diabetes is a prominent human pathology with increasing incidence in the population; however, its cause is still unknown. This disease promotes detrimental effects on reproduction, such as lower sperm motility and DNA integrity. Hence, the investigation of the underlying mechanisms of this metabolic disturbance in reproduction and its transgenerational consequences is of the utmost importance. The zebraf...
Zebrafish models to study ectopic calcification and calcium-associated pathologies
Santos, João; Laizé, Vincent; J. Gavaia, Paulo; Conceição, Natércia; Leonor Cancela, M.Ectopic calcification refers to the pathological accumulation of calcium ions in soft tissues and is often the result of a dysregulated action or disrupted function of proteins involved in extracellular matrix mineralization. While the mouse has traditionally been the go-to model organism for the study of pathologies associated with abnormal calcium deposition, many mouse mutants often have exacerbated phenotyp...
Antioxidant and anti-inflammatory extracts from sea cucumbers and tunicates ind...
Carletti, Alessio; Cardoso, Carlos; Lobo-Arteaga, Jorge; Sales, Sabrina; Juliao, Diana; Ferreira, Inês; Chainho, Paula; Dionísio, Maria AnaBone metabolic disorders such as osteoporosis are characterized by the loss of mineral from the bone tissue leading to its structural weakening and increased susceptibility to fractures. A growing body of evidence suggests that inflammation and oxidative stress play an important role in the pathophysiological processes involved in the rise of these conditions. As the currently available therapeutic strategies a...
Screening of mineralogenic and osteogenic compounds in zebrafish—tools to impro...
Rosa, Joana; Tarasco, Marco; J. Gavaia, Paulo; Leonor Cancela, M.; Laizé, VincentBone disorders affect millions of people worldwide and treatments currently available often produce undesirable secondary effects or have limited efficacy. It is therefore of the utmost interest for patients to develop more efficient drugs with reduced off-target activities. In the long process of drug development, screening and preclinical validation have recently gained momentum with the increased use of zebr...
Cells isolated from regenerating caudal fin of Sparus aurata can differentiate ...
Vijayakumar, Parameswaran; Cardeira, João; Laizé, Vincent; J. Gavaia, Paulo; Cancela, M. LeonorTeleosts have the ability to regenerate their caudal fin upon amputation. A highly proliferative mass of undifferentiated cells called blastema forms beneath wound epidermis and differentiates to regenerate all missing parts of the fin. To date, the origin and fate of the blastema is not completely understood. However, current hypotheses suggest that the blastema is comprised of lineage-restricted dedifferentia...
Microdiet formulation with phospholipid modulate zebrafish skeletal development...
Martins, Gil; Diogo, Patricia; Santos, Tamara; Cabrita, Elsa; Pinto, Wilson; Dias, Jorge; J. Gavaia, PauloDietary phospholipids' (PLs) content, origin, and profile are known to affect fish development and reproductive performance, but their effects in zebrafish (Danio rerio) nutrition are still poorly investigated. Therefore, this study aimed to assess the effect of practical microdiets containing plant-based and marine PL sources in zebrafish growth, survival, skeletal development, and reproductive performance. Re...
Reduction of skeletal anomalies in meagre (Argyrosomus regius, Asso, 1801) thro...
Martins, Gil; Ribeiro, Laura; Candeias-Mendes, Ana; Diogo, Patricia; Gamboa, Margarida; Barata, Marisa; Leonor Cancela, M.; Pousao-Ferreira, PedroThe consolidation of meagre (Argyrosomus regius) in aquaculture requires an understanding and optimization of larval rearing and nutritional conditions. The aim of this study was to analyse the effects of an early introduction of inert diets during larval rearing, on growth performance, digestive enzymes activity and development of skeletal anomalies. This study evaluated the effects of three different timings ...
Warfarin-exposed zebrafish embryos resembles human warfarin embryopathy in a do...
Granadeiro, Luis; Dirks, Ron P.; Ortiz-Delgado, Juan B.; J. Gavaia, Paulo; Sarasquete, Carmen; Laizé, Vincent; Leonor Cancela, M.; I, FernándezWarfarin is the most worldwide used anticoagulant drug and rodenticide. Since it crosses placental barrier it can induce warfarin embryopathy (WE), a fetal mortality in neonates characterized by skeletal deformities in addition to brain hemorrhages. Although the effects of warfarin exposure in aquatic off target species were already described, the particular molecular toxicological mechanisms during early devel...