9 documents found, page 1 of 1
Surfactin and polyglutamic acid coproduction by Bacillus velezensis P#02 using ...
Correia, Jéssica Alexandra Henriques; Gudiña, Eduardo José; Janek, Tomasz; Dias, Ricardo; Teixeira, J. A.; Freitas, Victor deBacillus velezensis P#02 simultaneously produced surfactin and polyglutamic acid (PGA). Among the different culture media studied, the one containing corn steep liquor (100 mL/L), glucose (10 g/L), and glutamic acid (10 g/L) as sole ingredients (CSLGGlut(10)) offered the best results regarding biosurfactant and biopolymer production. Although biosurfactant production occurred both under shaking and static condi...
Efficient conversion of black cumin cake from industrial waste into lipopeptide...
Ciurko, Dominika; Laba, Wojciech; Kancelista, Anna; John, Lukasz; Gudiña, Eduardo José; Lazar, Zbigniew; Janek, TomaszMost biosurfactants are obtained using costly culture media, which limits their wider industrial use. In the present study, a low-cost culture medium, containing the agro-industrial residue black cumin cake, was developed for amphisin production by Pseudomonas fluorescens DSS73. By using black cumin cake as the substrate, not only was the production cost reduced but also a higher production yield was achieved. ...
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using ...
Correia, Jéssica; Gudiña, Eduardo José; Lazar, Zbigniew; Janek, Tomasz; Teixeira, J. A.The agro-industrial by-products corn steep liquor (CSL) and olive mill wastewater (OMW) were evaluated as low-cost substrates for rhamnolipid production by Burkholderia thailandensis E264. In a culture medium containing CSL (7.5% (v/v)) as sole substrate, B. thailandensis E264 produced 175 mg rhamnolipid/L, which is about 1.3 times the amount produced in the standard medium, which contains glycerol, peptone, an...
Metal-biosurfactant complexes characterization: binding, self-assembly and inte...
Janek, Tomasz; Rodrigues, L. R.; Gudiña, Eduardo J.; Czyznikowska, ZanetaStudies on the specific and nonspecific interactions of biosurfactants with proteins are broadly relevant given the potential applications of biosurfactant/protein systems in pharmaceutics and cosmetics. The aim of this study was to evaluate the interactions of divalent counterions with the biomolecular anionic biosurfactant surfactin-C15 through molecular modeling, surface tension and dynamic light scattering ...
Effect of high pressure on surfactin production by Bacillus subtilis: implicati...
Gudiña, Eduardo José; Meneses, C.; Rodrigues, Ana Isabel Geraldes; Janek, Tomasz; Rodrigues, L. R.Surfactin, a lipopeptide biosurfactant produced by Bacillus subtilis strains, exhibits extraordinary surface active properties, as well as stability at a wide range of temperatures and salinities, making it useful to replace the chemical surfactants in many industrial applications. The oil industry can take advantage of its application to increase the productivity of oil reservoirs, through a technology known a...
Biomolecular interactions of lysosomotropic surfactants with cytochrome c and i...
Janek, Tomasz; Czelen, Przemyslaw; Gudiña, Eduardo José; Rodrigues, L. R.; Czyznikowska, ZanetaThe molecular interactions between two single-chain lysosomotropic surfactants DMM-11 (2-Dodecanoyloxyethyl)trimethylammonium bromide) and DMPM-11 (2-Dodecanoyloxypropyl)trimethylammonium bromide) with a small heme-protein (cytochrome c (cyt-c)) in Hepes buffer (pH = 7.4) were extensively investigated by surface tension, dynamic light scattering (DLS), circular dichroism (CD) and fluorescence spectroscopy in co...
Study of metal-lipopeptide complexes and their self-assembly behavior, micelle ...
Janek, Tomasz; Rodrigues, L. R.; Czyznikowska, ZanetaThe present study aimed to explore the interactions of divalent counterions with biomolecular amphisin using circular dichroism (CD), ultravioletvisible (UVVis) and density functional theory (DFT). The binding mode of interactions between metal-amphisin complexes and bovine serum albumin (BSA) were studied using fluorescence spectroscopy. The results showed that Cu2+ is coordinated by one oxygen atom of the asp...
Physicochemical study of biomolecular interactions between lysosomotropic surfa...
Janek, Tomasz; Czyznikowska, Zaneta; Luczynski, Jacek; Gudiña, Eduardo J.; Rodrigues, L. R.; Galezowska, JoannaThe interactions between two cationic lysosomotropic surfactants (2-dodecanoyloxyethyl)trimethylammonium bromide (DMM-11) and (2-dodecanoyloxypropyl)trimethylammonium bromide (DMPM-11) with bovine serum albumin (BSA) in Hepes buffer (pH = 7.4) were systematically studied by surface tension, fluorescence and circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC). Furthermore, the size of...
Structure and mode of action of cyclic lipopeptide pseudofactin II with divalen...
Janek, Tomasz; Rodrigues, L. R.; Gudiña, Eduardo J.; Czyznikowska, ZanetaThe interaction of natural lipopeptide pseudofactin II with a series of doubly charged metal cations was examined by matrixassisted laserdesorption ionizationtime of flight (MALDI-TOF) mass spectrometry and molecular modelling. The molecular modelling for metal-pseudofactin II provides information on the metalpeptide binding sites. Overall, Mg2+, Ca2+ and Zn2+ favor the association with oxygen atoms spanning th...