5 documents found, page 1 of 1
Stromal vascular fraction obtained from subcutaneous adipose tissue: ex-obese a...
Frias, Frias; Matos, Beatriz; Jarnalo, Mariana; Freitas-Ribeiro, Sara; Reis, R. L.; Pirraco, Rogério P.; Horta, RicardoIntroduction. Human adipose tissue contains a heterogeneous and synergistic mixture of cells called stromal vascular fraction (SVF) with highly proliferative and angiogenic properties, conferring promising applicability in the field of regenerative medicine. This study aims to investigate if age, body mass index (BMI), history of obesity and massive weight loss, and harvest site are related to SVF cell marker e...
Pre-selection of fibroblasts subsets prompt prevascularization of tissue engine...
Moreira, Helena R.; Cerqueira, Mariana T.; Silva, Lucília Pereira; Pires, Joana; Jarnalo, Mariana; Horta, Ricardo; Reis, R. L.; Marques, A. P.The papillary and reticular dermis harbors phenotypically distinct fibroblasts, whose functions such as maintenance of skin's microvasculature are also distinct. Thus, we hypothesized that pre-selection of the subpopulations of fibroblasts would benefit the generation of skin tissue engineered (TE) constructs, promoting their prevascularization in vitro. We first isolated papillary and reticular fibroblasts usi...
Spongy-like hydrogels prevascularization with the adipose tissue vascular fract...
Moreira, Helena R.; Rodrigues, Daniel Barreira; Ribeiro, Sara Freitas; Silva, Lucília Pereira; Morais, Alain da S.; Jarnalo, Mariana; Horta, RicardoIn vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important...
Integrin-specific hydrogels for growth factor-free vasculogenesis
Moreira, Helena R.; Rodrigues, Daniel Barreira; Ribeiro, Sara Freitas; Silva, Lucília Pereira; Morais, Alain da S.; Jarnalo, Mariana; Horta, RicardoIntegrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascu...
Growth factor-free vascularization of marine-origin collagen sponges using cryo...
Freitas-Ribeiro, Sara; Diogo, Gabriela S.; Oliveira, Catarina; Martins, Albino; Silva, Tiago H.; Jarnalo, Mariana; Horta, Ricardo; Reis, R. L.The successful integration of transplanted three-dimensional tissue engineering (TE) constructs depends greatly on their rapid vascularization. Therefore, it is essential to address this vascularization issue in the initial design of constructs for perfused tissues. Two of the most important variables in this regard are scaffold composition and cell sourcing. Collagens with marine origins overcome some issues a...