24 documents found, page 1 of 3
Fast calculation of spectral optical properties and pigment content detection i...
Botelho, Ana R.; Silva, Hugo F.; Martins, Inês S.; Carneiro, Isa; Carvalho, Sónia D.; Henrique, Rui M.; Tuchin, Valery V.; Oliveira, Luís M.A fast calculation method was used to obtain the spectral optical properties of human normal and pathological (chromophobe renal cell carcinoma) kidney tissues. Using total transmittance, total reflectance and collimated transmittance spectra acquired from ex vivo kidney samples, the spectral optical properties of both tissues, namely the absorption, the scattering and the reduced scattering coefficients, as we...
Gal-3 Protein Expression and Localization in Prostate Tumours
Lima, Tânia; Macedo-Silva, Catarina; Felizardo, Diana; Fraga, João; Carneiro, Isa; Jerónimo, Carmen; Henrique, Rui; Fardilha, Margarida; Vitorino, RuiGal-3 plays an important role in cell survival, mRNA splicing, and cell-cell and cell-matrix interactions. Depending on its cellular localization and cancer type, Gal-3 may have tumour-suppressive or tumour-promoting activities. Given the promising diagnostic role of Gal-3 in the urine of PCa patients found in our previous study, its concordant gene and protein expression levels, and its involvement in PCa-rela...
PP1 catalytic isoforms are differentially expressed and regulated in human pros...
Felgueiras, Juliana; Lobo, João; Camilo, Vânia; Carneiro, Isa; Matos, Bárbara; Henrique, Rui; Jerónimo, Carmen; Fardilha, MargaridaThe Ser/Thr-protein phosphatase PP1 (PP1) is a positive regulator of the androgen receptor (AR), which suggests major roles for PP1 in prostate carcinogenesis. However, studies dedicated to the characterization of PP1 in PCa are currently scarce. Here we analyzed the expression and localization of the PP1 catalytic (PP1c) isoforms in formalin-fixed, paraffin-embedded prostate tissue samples, as well as in PCa c...
Enhanced Ultraviolet Spectroscopy by Optical Clearing for Biomedical Applications
Carneiro, Isa; Carvalho, Sonia; Henrique, Rui; Selifonov, Alexey; Oliveira, Luís; Tuchin, Valery V.In this paper, we describe the combination of ultraviolet (UV) spectroscopy with the optical clearing technique to induce new tissue windows, evaluate their efficiency, study the diffusion properties of agents and discriminate cancer. The use of highly concentrated glycerol solutions has induced high efficiency clearing effects in the UV, both in human colorectal and gingival tissues. The protein dissociation r...
Diffuse reflectance and machine learning techniques to differentiate colorectal...
Fernandes, Luís; Carvalho, Sónia; Carneiro, Isa; Henrique, Rui; Tuchin, Valery V.; Oliveira, Hélder P.; Oliveira, LuísIn this study, we used machine learning techniques to reconstruct the wavelength dependence of the absorption coefficient of human normal and pathological colorectal mucosa tissues. Using only diffuse reflectance spectra from the ex vivo mucosa tissues as input to algorithms, several approaches were tried before obtaining good matching between the generated absorption coefficients and the ones previously calcul...
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of...
Fernandes, Luís; Silva, Hugo; Martins, Inês; Carvalho, Sónia; Carneiro, Isa; Henrique, Rui; Tuchin, Valery V.; Oliveira, LuísIn this paper we present three studies that demonstrate the applicability of spectroscopy methods and optical clearing treatments in pathology identification and monitoring. In the first study, by obtaining the absorption spectra of human healthy and pathological (adenocarcinoma) colorectal mucosa tissues, it was possible to identify a higher content of a pigment in the diseased tissues. This study also shows t...
Lipofuscin-Type Pigment as a Marker of Colorectal Cancer
Carvalho, Sónia; Carneiro, Isa; Henrique, Rui; Tuchin, Valery; Oliveira, LuísThe study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method, and with the exception of the reduced scatt...
Measurement of optical properties of normal and pathological human liver tissue...
Carneiro, Isa; Carvalho, Sónia; Henrique, Rui; Oliveira, Luís; Tuchin, Valery V.The interest of using light in clinical practice is increasing strongly and many applications work at various wavelengths from the ultraviolet to the infrared. Due to this great range of applications, the determination of the optical properties of biological tissues in a wide spectral range becomes of interest. The liver is an important organ, since it has a major role in the human body and various pathologies ...
Método de criação de janelas óticas de diagnóstico e tratamento em materiais bi...
Oliveira, Luís; Carneiro, Isa; Carvalho, Sónia; Henrique, Rui; Tuchin, ValeryPatente nacional submetida a março de 2019 e publicada a 14 de setembro de 2020. PT115371 (A); A invenção fornece uma diminuição na dispersão de luz e um maior aumento na transparência do tecido na região do UV em comparação com as regiões do visível e infravermelho. 12 p.p. f-ly, 5 ex., 7 ill.
Measuring optical properties of human liver between 400 and 1000 nm
Carneiro, Isa; Carvalho, Sónia; Henrique, Rui; Oliveira, Luís; Tuchin, ValeryLaser diagnostics and treatment procedures are commonly performed for visible and near-IR wavelengths. The knowledge of the wavelength dependences for the optical properties of various biological tissues in this spectral range is useful for clinical applications. Since the optical properties of human liver have been previously known only for near-IR wavelengths, the aim is to estimate their wavelength dependenc...