6 documents found, page 1 of 1
Encapsulated laccase in bimetallic Cu/Zn ZIFs as stable and reusable biocatalys...
Yang, Xue; Zhao, Jing; Cavaco-Paulo, Artur; Jing Su; Wang, HongboLaccase have received extensive attention in pollutant degradation, but its practical viability is largely affected by the poor stability, easy inactivation and difficulty in recycling for the present. Enzyme immobilization offers enhanced enzyme stability and constructs a synergistic system for the efficient adsorption and degradation of pollutants. In this study, bimetallic Cu/Zn ZIFs were synthesized by co-p...
Antimicrobial coating of textiles by laccase in situ polymerization of catechol...
Jing Su; Jennifer Noro; Silva, Sónia Carina; Fu, Jiajia; Qiang Wang; Ribeiro, Artur; Silva, Carla; Cavaco-Paulo, ArturTextile fabrics made up of cotton, wool, and polyethylene terephthalate (PET) were coated with poly(catechol) and poly(p-phenylenediamine) through the in situ enzymatic polymerization of catechol and p-phenylenediamine, assisted by laccase under high-pressure homogenization. All coated fabrics showed antimicrobial activity against both gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) s...
Exploring PEGylated and immobilized laccases for catechol polymerization
Jing Su; Noro, Jennifer Martins; Fu, Jiajia; Wang, Qiang; Silva, Carla; Cavaco-Paulo, ArturLaccases have been reported for their ability to eliminate hazardous phenolic compounds by oxidative polymerization. The exploitation of the oxidative behavior of different laccase forms, namely free/native, free/PEGylated, immobilized/native and immobilized/PEGylated, was assessed in this study. We found that PEGylated and immobilized laccase forms have differentiated catalytic behavior revealing distinct conv...
Practical insights on enzyme stabilization
Silva, Carla; Sá, M.; Jing Su; Fu, Jiajia; Cavaco-Paulo, ArturEnzymes are efficient catalysts designed by nature to work in physiological environments of living systems. The best operational conditions to access and convert substrates at the industrial level are different from nature and normally extreme. Strategies to isolate enzymes from extremophiles can redefine new operational conditions, however not always solving all industrial requirements. The stability of enzyme...
Laccase: a green catalyst for the biosynthesis of poly-phenols
Jing Su; Fu, Jiajia; Wang, Qiang; Silva, Carla Manuela Pereira Marinho; Cavaco-Paulo, ArturLaccases (benzene diol: oxidoreductases, EC 1.10.3.2) are able to catalyze the oxidation of various compounds containing phenolic and aniline structures using dissolved oxygen in water. Laccase structural features and catalytic mechanisms focused on the polymerization of aromatic compounds are reported. A description about the most recent research on the biosynthesis of chemicals and polymers is made. Selected ...
The effect of high-energy environments on the structure of laccase-polymerized ...
Jing Su; Castro, T.; Noro, Jennifer Martins; Fu, Jiajia; Wang, Qiang; Silva, C.; Cavaco-Paulo, ArturThe laccase polymerization of catechol was performed using different reactors namely a water bath (WB), an ultrasonic bath (US) and a high-pressure homogenizer (HPH). The total content of free OH and the MALDI-TOF spectra of polymers obtained demonstrated that reactions are favored in the presence of high-energy environments. Higher conversion yields and polymerization degrees (DP) were obtained after polymeriz...