21 documents found, page 1 of 3

Distribution of Chlamydia trachomatis ompA-genotypes over three decades in Port...

Objectives: Chlamydia trachomatis is classified into 15 major genotypes, A to L3, based on the diversity of ompA gene. Here, we evaluated and characterised the distribution and diversity of ompA-genotypes over 32 years (1990–2021) in Portugal. Methods: The collection of the Portuguese National Reference Laboratory for Sexually Transmitted Infections includes 5824 C. trachomatis-positive samples that were succes...

Surveying genetic markers of antibiotic resistance and genomic background in Ch...

Objectives: To survey genetic markers of potential antimicrobial resistance (AMR) to macrolides and fluoroquinolones among Chlamydia trachomatis–positive samples from the collection of the Portuguese National Reference Laboratory for Sexually Transmitted Infections (STIs), and explore a multiplex PCR approach coupled with NGS to provide complementary information regarding a strain’s genomic backbone. Methods: A...

Lymphogranuloma venereum (LGV) ompA-subvariants of the Portuguese collection of...

Background: Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis ompA-genotypes L1–L3, with increasing numbers of detected cases across Europe. Here, we analysed diversity and temporal distribution of the LGV ompA-subvariants detected in Portugal between 2007 and 2023, in order to better understand the dissemination and diversification landscape of LGV strains. Meth...

Infeções por Chlamydia trachomatis e Neisseria gonorrhoeae: resultados do diagn...

Chlamydia trachomatis e Neisseria gonorrhoeae são responsáveis pelas duas infeções sexualmente transmissíveis (IST) curáveis mais comuns em todo o mundo. Sendo frequentemente assintomáticas, o rastreio laboratorial destas IST é fundamental, para que possa ser efetuado o tratamento adequado que evite o desenvolvimento de sequelas clínicas graves e para quebrar cadeias de transmissão. O presente estudo teve por o...

Orogenital and anal infection by Chlamydia trachomatis, Neisseria gonorrhoeae, ...

Background: Men who have sex with men (MSM) are at risk for sexually transmitted infections (STIs), but more data on extragenital carriage are needed. Aim: We assessed the genital and extragenital prevalence of bacterial and other STIs in MSM in a Lisbon sexual health clinic. Methods: We screened oral, anal, and urine samples of MSM visiting the GAT-CheckpointLX clinic June 2017-December 2021 for Chlamydia trac...

Use of the trapeze bar to support mobility - A nursing perspective

Background: assistive devices, as the trapeze bar, are designed to enhance functional capacity of individuals with deficits and improve/maintain their physical functionality and independence in daily activities, as transfers and ambulation. Objectives: to characterize the patient's level of dependency, to ascertain if nursing interventions are adjusted to patient's needs, and to determine if provision and use o...

On nontuberculous mycobacteria in human alveolar macrophages

Background: Despite growing relevance of nontuberculous mycobacteria (NTM) infections, namely pulmonary infections, accurate data on incidence and pathogenesis is lacking. Iintracellular persistence of three NTM species in a human alveolar macrophages model. The contribution of particular innate immune mechanisms and NTM genomes to mycobacteria intracellular fate were investigated. Materials/methods: M.smegmati...

Nontuberculous Mycobacteria Persistence in a Cell Model Mimicking Alveolar Macr...

Nontuberculous Mycobacteria (NTM) respiratory infections have been gradually increasing. Here, THP-1 cells were used as a model to evaluate intracellular persistence of three NTM species (reference and clinical strains) in human alveolar macrophages. The contribution of phagosome acidification, nitric oxide (NO) production and cell dead on NTM intracellular fate was assessed. In addition, strains were character...

General Overview on Nontuberculous Mycobacteria, Biofilms, and Human Infection

Nontuberculous mycobacteria (NTM) are emergent pathogens whose importance in human health has been growing. After being regarded mainly as etiological agents of opportunist infections in HIV patients, they have also been recognized as etiological agents of several infections on immune-competent individuals and healthcare-associated infections. The environmental nature of NTMand their ability to assemble biofilm...

Vigilância laboratorial das infeções por Neisseria gonorrhoeae em Portugal, 200...