59 documents found, page 1 of 6
Cell factories for sustainable production of valuable chemicals – the pivotal r...
Silva, Paulo César; Johansson, BjörnThe green economy aims to substitute polluting chemicals and processes with sustainable counterparts deriving from microbial cell factories that provide catalytic specificity and efficient conversions. In this manuscript, we explore how the bio-based production of chemicals enables the production of valuable molecules while reducing contemporary society's dependence on fossil fuels thereby alleviating part of t...
Cell factories for sustainable production of valuable chemicals – the pivotal r...
Silva, Paulo César; Johansson, BjörnThe green economy aims to substitute polluting chemicals and processes with sustainable counterparts deriving from microbial cell factories that provide catalytic specificity and efficient conversions. In this manuscript, we explore how the bio-based production of chemicals enables the production of valuable molecules while reducing contemporary society's dependence on fossil fuels thereby alleviating part of t...
Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase sys...
Pozdniakova, T. A.; Cruz, João P.; Silva, Paulo César; Azevedo, Flávio; Parpot, Pier; Domingues, Maria Rosario; Carlquist, Magnus; Johansson, BjörnFatty acids are produced by eukaryotes like baker’s yeast Saccharomyces cerevisiae mainly using a large multifunctional type I fatty acid synthase (FASI) where seven catalytic steps and a carrier domain are shared between one or two protein subunits. While this system may offer efficiency in catalysis, only a narrow range of fatty acids are produced. Prokaryotes, chloroplasts and mitochondria rely instead on a ...
Bacteria and yeast colony PCR
Pereira, Humberto; Silva, Paulo César; Johansson, BjörnThe bacteria Escherichia coli and the yeast Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E. coli is almost always used for fundamental DNA manipulation, while yeast is the simplest host system for studying eukaryotic gene expression and performing large-scale DNA assembly. Yeast expression studies may also require altering the chromosomal DNA by homologous recomb...
Relativism in the Cloud: Cloud Sourcing in virtue of IS Development Outsourcing...
Johansson, Björn; Muhic, MirellaNowadays Cloud Computing and Cloud Sourcing is on the agenda in many organizations. Many Chief Information Officers (CIOs) that urge for alternatives to traditional outsourcing are interested in how they can take advantage from Cloud Computing, by sourcing Information Technology (IT) from the cloud. This paper provides an overview of the research direction of Cloud Sourcing in the IS field. A literature review ...
Sourcing motives behind sourcing decisions exposed through the Sourcing Decisio...
Muhic, Mirella; Johansson, BjörnThere is no doubt that information systems (IS) are the backbone of today’s organizations. Having an initial inspection on sourcing motives in the financial sector it can be stated that resources used in development of information systems (IS) are seen as an important factor for sustained competitive advantage. However, it can be claimed that it depends to a high extent on the application of different sourcing ...
Getting the balance right between functional and non-functional requirements: t...
Johansson, Björn; Lahtinen, MarkusIT procurement represents a business process of high importance, including the ability to articulate requirements that the procurement deals with. Furthermore, specifying requirements is of importance for both procurer and potential supplier, as it functions as central contractual element between the two. The purpose of this article is two-fold: (i) to show how established terminology for requirement specificat...
Expression of Yarrowia lipolytica acetyl-CoA carboxylase in Saccharomyces cerev...
Pereira, Humberto; Azevedo, Flávio; Domingues, Lucília; Johansson, BjörnMalonyl-CoA is an energy-rich molecule formed by the ATP-dependent carboxylation of acetyl coenzyme A catalyzed by acetyl-CoA carboxylase. This molecule is an important precursor for many biotechnologically interesting compounds such as flavonoids, polyketides, and fatty acids. The yeast Saccharomyces cerevisiae remains one of the preferred cell factories, but has a limited capacity to produce malonyl-CoA compa...
Towards engineered yeast as production platform for capsaicinoids
Muratovska, N.; Silva, P.; Pozdniakova, T. A.; Pereira, H.; Grey, C.; Johansson, Björn; Carlquist, M.Capsaicinoids are bioactive alkaloids produced by the chili pepper fruit and are known to be the most potent agonists of the human pain receptor TRPV1 (Transient Receptor Potential Cation Channel Subfamily V Member 1). They are currently produced by extraction from chili pepper fruit or by chemical synthesis. Transfer of the biosynthetic route to a microbial host could enable more efficient capsaicinoid product...
Relativism in the Cloud: Cloud Sourcing in virtue of IS Development Outsourcing...
Johansson, Björn; Muhic, MirellaNowadays Cloud Computing and Cloud Sourcing is on the agenda in many organizations. Many Chief Information Officers (CIOs) that urge for alternatives to traditional outsourcing are interested in how they can take advantage from Cloud Computing, by sourcing Information Technology (IT) from the cloud. This paper provides an overview of the research direction of Cloud Sourcing in the IS field. A literature review ...