6 documents found, page 1 of 1
Mitochondria-targeted anti-oxidant AntiOxCIN4 improved liver steatosis in Weste...
Amorim, Ricardo; Simões, Inês C. M.; Teixeira, José; Cagide, Fernando; Potes, Yaiza; Soares, Pedro; Carvalho, Adriana; Tavares, Ludgero C.Non-alcoholic fatty liver disease (NAFLD) is a health concern affecting 24% of the population worldwide. Although the pathophysiologic mechanisms underlying disease are not fully clarified, mitochondrial dysfunction and oxidative stress are key players in disease progression. Consequently, efforts to develop more efficient pharmacologic strategies targeting mitochondria for NAFLD prevention/treatment are underw...
Estimating pentose phosphate pathway activity from the analysis of hepatic glyc...
Belew, Getachew D.; Di Nunzio, Giada; Tavares, Ludgero; Silva, Joao G.; Torres, Alejandra N.; Jones, John GriffithThe pentose phosphate pathway (PPP) is an important component of hepatic intermediary metabolism. Jin et al developed an elegant 13 C-NMR method for measuring hepatic PPP flux by quantifying the distribution of glucose 13 C-isotopomers formed from [U-13 C]glycerol. We demonstrate that this approach can be extended to exogenous [U-13 C]fructose and [U-13 C]glucose precursors by 13 C-NMR analysis of glycogen.; Re...
Limitations to Starch Utilization in Barramundi (Lates calcarifer) as Revealed ...
Palma, Mariana; Trenkner, Lauren H.; Rito, João; Tavares, Ludgero C.; Silva, Emanuel; Glencross, Brett D.; Jones, John Griffith; Wade, Nicholas M.Practical diets for commercial barramundi production rarely contain greater than 10% starch, used mainly as a binding agent during extrusion. Alternative ingredients such as digestible starch have shown some capacity to spare dietary protein catabolism to generate glucose. In the present study, a carnivorous fish species, the Asian seabass (Lates calcarifer) was subjected to two diets with the same digestible e...
Sources of hepatic glycogen synthesis in mice fed with glucose or fructose as t...
Jarak, Ivana; Barosa, Cristina; Martins, Fátima O.; Silva, Joao C. P.; Santos, Cristiano; Belew, Getachew Debas; Rito, João; Viegas, IvanThe positional analysis of hepatic glycogen enrichment from deuterated water (2 H2 O) by 2 H NMR has been applied previously to resolve the contributions of glucose and fructose to glycogen synthesis in rodents fed a high sucrose diet. To further validate this method, this analysis was applied to mice fed with synthetic diets whose carbohydrate components consisted solely of either glucose or fructose.; The aut...
A Mitochondrial Approach to Cardiovascular Risk and Disease
Veloso, Caroline D.; Belew, Getachew D.; Ferreira, Luciana L.; Grilo, Luís F.; Jones, John Griffith; Portincasa, Piero; Sardão, Vilma A.Cardiovascular diseases (CVDs) are a leading risk factor for mortality worldwide and the number of CVDs victims is predicted to rise through 2030. While several external parameters (genetic, behavioral, environmental and physiological) contribute to cardiovascular morbidity and mortality; intrinsic metabolic and functional determinants such as insulin resistance, hyperglycemia, inflammation, high blood pressure...
Estimating gluconeogenesis by NMR isotopomer distribution analysis of [13 C]bic...
Alves, Tiago Cardoso; Nunes, Patrícia Maria; Palmeira, Carlos Marques; Jones, John Griffith; Carvalho, Rui AlbuquerqueThe gluconeogenic contribution to glucose production in livers isolated from rats fasted for 24 h was determined by 13C-NMR isotopomer distribution analysis of secreted glucose enriched from 99% [13C]bicarbonate (n = 4) and 99% [1-13C]lactate (n = 4). Experiments with 3% 2H2O were also performed, allowing the gluconeogenic contribution to be measured by the relative 2H enrichments at positions 5 and 2 of glucos...