53 documents found, page 1 of 6
Plasmid-Mediated Colistin Resistance (mcr-1) in Escherichia coli from Non-Impor...
Manageiro, Vera; Jones-Dias, Daniela; Ferreira, Eugénia; Caniça, ManuelaIn this study, we report the presence of the plasmid-mediated colistin resistance (PMCR)-encoding gene mcr-1 in an Escherichiacoli isolate, INSali25, recovered from lettuce produced and marketed in Portugal. Colistin MIC from the vegetable E. coli isolate-determined by microdilution broth method according to EUCAST guidelines-revealed a non-wild-type phenotype of colistin (MIC 16 mg/L). To understand the geneti...
Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Cl...
Manageiro, Vera; Félix, David; Jones-Dias, Daniela; Sampaio, Daniel A; Vieira, Luís; Sancho, Luísa; Ferreira, Eugénia; Caniça, ManuelaA new QepA4 variant was detected in an O86:H28 ST156-fimH38 Escherichia coli, showing a multidrug-resistance phenotype. PAβN inhibition ofqepA4-harboring transconjugant resulted in increase of nalidixic acid accumulation. TheqepA4andcatA1genes were clustered in a 26.0-kp contig matching an IncF-type plasmid, and containing a Tn21-type transposon with multiple mobile genetic elements. This QepA variant is worris...
Quantitative proteome analysis of an antibiotic resistant Escherichia coli expo...
Jones-Dias, Daniela; Carvalho, Ana Sofia; Moura, Inês Barata; Manageiro, Vera; Igrejas, Gilberto; Caniça, Manuela; Matthiesen, RuneTetracyclines are among the most commonly used antibiotics administrated to farm animals for disease treatment and prevention, contributing to the worldwide increase in antibiotic resistance in animal and human pathogens. Although tetracycline mechanisms of resistance are well known, the role of metabolism in bacterial reaction to antibiotic stress is still an important assignment and could contribute to the un...
Quantitative proteome analysis of an Escherichia coli exposed to tetracycline r...
Jones-Dias, Daniela; Carvalho, Ana Sofia; Moura, Inês Barata; Dill, Brian; Molina, Henrik; Manageiro, Vera; Caniça, Manuela; Matthiesen, RuneBackground: Many studies have already focused on the evaluation of variations in protein expression caused by antibiotic exposure in tetracycline resistant microorganisms. However, little is known about the metabolic response of genetically resistant bacterial populations to antibiotic exposure. In this study we used a liquid chromatography-mass spectrometry-based (LC-MS/MS) proteomics to evaluate the global cy...
Antibiotic-resistant bacteria from air samples collected in nursing homes
Salgueiro, Vanessa; Manageiro, Vera; Jones-Dias, Daniela; Cano, Manuela; Ferreira, Eugénia; Caniça, ManuelaIntroduction: Antibiotic resistance is currently a worldwide public health problem that can include diverse mechanisms and bacterial species. The understanding and monitoring of such phenomena is essential to avoid further dissemination. The main aim of this study was the characterization of antibiotic resistance mechanisms in bacterial strains collected in air samples from nursing homes and compare them with c...
Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recover...
Jones-Dias, Daniela; Manageiro, Vera; Ferreira, Eugénia; Barreiro, Paula; Vieira, Luís; Moura, Inês B; Caniça, ManuelaThe spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. To understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance Gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, to...
Assessing antibiotic resistance in Gram negative bacteria from animals and the ...
Jones-Dias, DanielaThe alarming increase in the levels of antibiotic resistant bacteria in clinical practice launched the call for a broader understanding of this event. This Ph.D. thesis aimed to unravel the main mobile antibiotic resistance determinants circulating in Gram negative bacteria from non-human sources, showing the contribution of mobile genetic elements for the overall process. The susceptibility and molecular epide...
New Class 2 Integron In2-4 Among IncI1-Positive Escherichia coli Isolates Carry...
Jones-Dias, Daniela; Manageiro, Vera; Martins, Ana P.; Ferreira, Eugénia; Caniça, ManuelaThe impact of extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (PMAβs) of animal origin constitutes a public health concern. In this study, 179 Escherichia coli from food animals and products were analyzed, among which, 15 cephalosporin-resistant isolates harboring ESBL (CTX-M-1 [n = 8], CTX-M-14 [n = 1], SHV-12 [n = 2]) or PMAβ [CMY-2, n = 5]) were identified in poultry and swine, ...
Salmonella Enteritidis Isolate Harboring Multiple Efflux Pumps and Pathogenicit...
Jones-Dias, Daniela; Clemente, Lurdes; Egas, Conceição; Froufe, Hugo; Sampaio, Daniel A; Vieira, Luís; Fookes, Maria; Thomson, Nicholas RBACKGROUND: Salmonella enterica is one of the most important causes of gastrointestinal infection in humans, being the great majority of infections related to the consumption of poultry meat and eggs (Foley and Lynne, 2008; EFSA/ECDC, 2015). In animals, infections caused by serotype Enteritidis are rarely responsible for severe disease with animals frequently becoming asymptomatic carriers, except in the case o...
Draft Genomic Analysis of an Avian Multidrug Resistant Morganella morganii Isol...
Jones-Dias, Daniela; Clemente, Lurdes; Moura, Inês B; Sampaio, Daniel A; Albuquerque, Teresa; Vieira, Luís; Manageiro, Vera; Caniça, ManuelaMorganella morganii is a commensal bacterium and opportunistic pathogen often present in the gut of humans and animals. We report the 4.3 Mbp draft genome sequence of a M. morganii isolated in association with an Escherichia coli from broilers in Portugal that showed macroscopic lesions consistent with colisepticemia. The analysis of the genome matched the multidrug resistance phenotype and enabled the identifi...