22 documents found, page 1 of 3
Discerning the role of polymicrobial biofilms in the ascent, prevalence, and ex...
Lopes, Susana Patrícia; Jorge, Paula Alexandra Silva; Sousa, Ana Margarida; Pereira, Maria OlíviaAntimicrobial therapy is facing a worrisome and underappreciated challenge, the phenomenon of heteroresistance (HR). HR has been gradually documented in clinically relevant pathogens (e.g. Pseudomonas aeruginosa, Staphylococcus aureus, Burkholderia spp., Acinetobacter baumannii, Klebsiella pneumoniae, Candida spp.) towards several drugs and is believed to complicate the clinical picture of chronic infections. T...
Biopolymer films doped with silver(I) coordination polymers against bacterial b...
Fernandes, T. A.; Costa, I. F. M.; Jorge, Paula Alexandra Silva; Sousa, A. C.; André, V.; Cerca, Nuno; Kirillov, A. M.This report describes a template-mediated self-assembly synthesis, full characterization, and structural features of two new silver-based bioactive coordination polymers (CPs) as well their immobilization into acrylated epoxidized soybean oil (ESOA) biopolymer films for antimicrobial applications. The 3D silver(I) CPs [Ag4(8-H2pma)2]n·4nH2O (1) and [Ag5(6-H0.5tma)2(H2O)4]n·2nH2O (2) were generated from AgNO3 an...
Silver(I) coordination polymers immobilized into biopolymer films for antimicro...
Fernandes, Tiago A.; Costa, Inês F. M.; Jorge, Paula Alexandra Silva; Sousa, Ana Catarina; André, Vânia; Cerca, Nuno; Kirillov, Alexander M.This study describes a template-mediated self-assembly synthesis, full characterization, and structural features of two new silver-based bioactive coordination polymers (CPs) and their immobilization into acrylated epoxidized soybean oil (ESOA) biopolymer films for antimicrobial applications. The 3D silver(I) CPs [Ag4(?8-H2pma)2]n·4nH2O (1) and [Ag5(?6-H0.5tma)2(H2O)4]n·2nH2O (2) were generated from AgNO3 and p...
Initial screening of poly(ethylene glycol) amino ligands for affinity purificat...
Silva, Nuno Miguel Sampaio Ribeiro Magalhães; Jorge, Paula Alexandra Silva; Martins, José A.; Teixeira, J. A.; Marcos, João C.Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing, which is mainly chromatography-based, of pDNA remains the key manufacturing step. Despite its high resolution, th...
Catalysing the way towards antimicrobial effectiveness: A systematic analysis a...
Jorge, Paula Alexandra Silva; Alves, Diana; Pereira, Maria OlíviaGrowing antimicrobial resistance and biofilm infection resilience have led researchers to study the potential laying in antimicrobial combinations, including those encompassing enzymes with biofilm disrupting abilities. Therefore, this work set out to evaluate the undergone journey of antimicrobial enzyme combination research and gain insights into its current status and most promising leads. Expert curators an...
Exploring anti-quorum sensing and anti-virulence based strategies to fight Cand...
Graínha, Tânia Raquel Rodrigues; Jorge, Paula Alexandra Silva; Pérez-Pérez, Martín; Rodríguez, Gael Pérez; Pereira, Maria OlíviaThe complex virulence attributes of Candida albicans are an attractive target to exploit in the development of new antifungals and anti-virulence strategies to combat C. albicans infections. Particularly, quorum-sensing (QS) has been reported as critical for virulence regulation in C. albicans. This work presents two knowledge networks with up-to-date information about QS regulation and experimentally tested an...
Antimicrobial peptide combinations against major infectious pathogens: in vitro...
Jorge, Paula Alexandra SilvaTese de Doutoramento - Engenharia Química e Biológica; Microbiology research has been investing significant efforts in the development of new and effective antimicrobial therapies against the growing number of multiresistant pathogens. Notably, the use of natural compounds, such as antimicrobial peptides (AMP), and the combination of different antimicrobials are being increasingly explored as means to improve i...
Effectiveness of colistin-antimicrobial peptides combinations against Pseudomon...
Jorge, Paula Alexandra Silva; Grzywacz, D.; Kamysz, W.; Lourenço, Anália Maria Garcia; Pereira, Maria OlíviaAntimicrobial research is increasingly being focused on the problem of microbial resistance and biofilm formation. Alternative mechanisms of action, as those of antimicrobial peptides (AMP), and synergic antimicrobial combinations can lead to more effective therapeutic strategies for this ever-growing world-wide problem. Hence, this work aimed to evaluate the antimicrobial and cytotoxic potential of combination...
Insights into Pseudomonas aeruginosa quorum sensing therapeutics through text- ...
Jorge, Paula Alexandra Silva; Pérez-Pérez, Martín; Rodríguez, Gael Pérez; Pereira, Maria Olívia; Lourenço, Anália Maria GarciaThe unceasing emergence of antibiotic-resistant strains and resilient biofilm-related infections is pressing researchers to develop novel antimicrobial strategies. Quorum sensing (QS) is a key communication mechanism that holds promise as target to control clinical pathogens. QS allows bacteria to regulate gene expression, and thus many physiological activities, such as virulence, motility, and biofilm formatio...
Developing timely insights into Pseudomonas aeruginosa quorum sensing therapeut...
Jorge, Paula Alexandra Silva; Pérez-Pérez, Martín; Pérez Rodríguez, Gael; Fdez Riverola, Florentino; Pereira, Maria Olívia; Lourenço, AnáliaPublicado em "Biofilms7: microbial works of art: book of abstracts". ISBN 978-989-97478-7-6; The pervasive growth of antibiotic-resistant is pressing the development of novel strategies to control infectious diseases. Quorum sensing (QS) is a key communication mechanism that allows bacteria to regulate gene expression, and thus many physiological activities e.g. virulence, motility, and biofilm formation. Hence...