7 documents found, page 1 of 1
Bioprospection of the bacterial β-myrcene-biotransforming trait in the rhizosphere
Castro, Pedro Miguel Soares; Soares, Filipa; Reis, Francisca Rodrigues; Lino-Neto, Teresa; Santos, Pedro M.The biocatalysis of β-myrcene into value-added compounds, with enhanced organoleptic/therapeutic properties, may be performed by resorting to specialized enzymatic machinery of β-myrcene-biotransforming bacteria. Few β-myrcene-biotransforming bacteria have been studied, limiting the diversity of genetic modules/catabolic pathways available for biotechnological research. In our model Pseudomonas sp. strain M1, t...
Current advances in the bacterial toolbox for the biotechnological production o...
Castro, Pedro Miguel Soares; Soares, Filipa; Santos, P. M.Monoterpenes are plant secondary metabolites, widely used in industrial processes as precursors of important aroma compounds, such as vanillin and (−)-menthol. However, the physicochemical properties of monoterpenes make difficult their conventional conversion into value-added aromas. Biocatalysis, either by using whole cells or enzymes, may overcome such drawbacks in terms of purity of the final product, ecolo...
Palatability of the Asian clam Corbicula fluminea (Müller 1774) in an invaded s...
Castro, Pedro Miguel Soares; Ilarri, M. I.; Modesto, V.; Antunes, C.; Sousa, Ronaldo GomesBiotic interactions such as predation may influence the success of invasive alien species (IAS). Although the Asian clam Corbicula fluminea is recognized as one of the most problematic aquatic IAS, few studies have investigated its use as a food resource. In this study, we aimed to investigate (i) the palatability of C. fluminea under natural (Minho River) and laboratory conditions and (ii) if C. fluminea submi...
Optimization of Pseudomonas sp. M1 as a biocatalyst of β-myrcene and related pl...
Castro, Pedro Miguel SoaresTese de Doutoramento em Biologia Molecular e Ambiental (Especialização em Biotecnologia Molecular); Several monoterpenes (e.g. β-myrcene) have a great industrial potential due to their organoleptic and therapeutic properties. However, monoterpene exploitation as industrially-relevant molecules is often limited because of their intricate properties (e.g. high hydrophobicity and structural instability). Biocataly...
Functional characterization of a 28-Kilobase Catabolic Island from Pseudomonas ...
Castro, Pedro Miguel Soares; Silva, Pedro Montenegro; Heipieper, Hermann J.; Santos, Pedro M.Pseudomonas sp. strain M1 is able to mineralize highly hydrophobic and recalcitrant compounds, such as benzene, phenol, and their methylated/halogenated derivatives, as well as the backbone of several monoterpenes. The ability to use such a spectrum of compounds as the sole carbon source is, most probably, associated with a genetic background evolved under different environmental constraints. The outstanding pe...
Deciphering the genome repertoire of Pseudomonas sp. M1 toward β-Myrcene biotra...
Castro, Pedro Miguel Soares; Santos, Pedro M.Pseudomonas sp. M1 is able to mineralize several unusual substrates of natural and xenobiotic origin, contributing to its competence to thrive in different ecological niches. In this work, the genome of M1 strain was resequenced by Illumina MiSeq to refine the quality of a published draft by resolving the majority of repeat-rich regions. In silico genome analysis led to the prediction of metabolic pathways invo...
Towards the description of the genome aatalogue of Pseudomonas sp. Strain M1
Castro, Pedro Miguel Soares; Santos, Pedro M.Pseudomonas sp. strain M1 is a soil isolate with remarkable biotechnological potential. The genome of Pseudomonas sp. M1 was sequenced using both 454 and Illumina technologies. A customized genome assembly pipeline was used to reconstruct its genome sequence to a single scaffold.; This work was supported by FEDER through POFC—COMPETE and by national funds from FCT through the projects PEst-C/BIA/UI4050/2011 and...