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Intermediate-tier options in the environmental risk assessment of plant protect...

Ernst, Gregor; Amorim, Mónica J. B.; Bottoms, Melanie; Brooks, Amy C; Hodson, Mark E; Kimmel, Stefan; Kotschik, Pia; Marx, Michael T

The European environmental risk assessment (ERA) of plant protection products follows a tiered approach. The approach for soil invertebrates currently consists of two steps, starting with a Tier 1 assessment based on reproduction toxicity tests with earthworms, springtails, and predatory mites. In case an unacceptable risk is identified at Tier 1, field studies can be conducted as a higher-tier option. For soil...


Environmental hazards of nanopesticides to non-target soil species - commercial...

Gomes, Susana I. L.; Chidiamassamba, Sekerani B.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

Nanopesticides (Npes) carry the potential of increased efficacy while reducing application rates, hence increasing agricultural productivity in a more sustainable way. However, given its novelty, the environmental risk assessment of these advanced materials is mostly absent. In the present study we investigated the ecotoxicity of a commercial insecticide, with reported nanofeatures, Karate Zeon®, and compared i...


Confirmatory assays for transient changes of omics in soil invertebrates - copp...

Bicho, Rita C.; Faustino, A. M. R.; Rêma, A.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

Environmental risk assessment (ERA) based on effects caused by chronic and longer term exposure is highly relevant. Further, if mechanistic based approaches (e.g. omics) can be included, beyond apical endpoints (e.g. reproduction), the prediction of effects increases. For Cu NMs (and CuCl2) this has been studied in detail, covering multi-omics and apical effects using the soil standard species Enchytraeus crypt...


Toxicity of boron and vanadium nanoparticles on Danio rerio embryos - phenotypi...

Santos, Joana; Barreto, Ângela; Almeida, Célia; Azevedo, Cátia; Domingues, Inês; Amorim, Mónica J. B.; Maria, Vera L.

Engineered nanoparticles (NPs) are emerging contaminants of concern and it is important to understand their environmental behavior and ecological risks to exposed organisms. Despite their ubiquitous presence in the environment, there is little information about the hazards of certain NPs, such as boron (BNPs) and vanadium (VNPs). The aim of the present research was to investigate the effects of commercial BNPs ...


Toxicity of fungicides to terrestrial non-target fauna - formulated products ve...

Gomes, Susana I. L.; Ammendola, Anna; Casini, Silvia; Amorim, Mónica J. B.

Despite the high usage of pesticides in current agricultural practices, its effects to humans and to the environment (non-target species) are a continuous concern. Soil dwelling organisms are among the first in line of exposure to pesticides, however their risks are often based on the pure active ingredient (a.i.) and not on the commercial formulated products (FPs) actually applied in the fields. In the present...


Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at populat...

Mendes, Luís André; Maria, Vera L.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effec...


Oxidative stress mechanisms caused by ag nanoparticles (NM300K) are different f...

Ribeiro, Maria J.; Maria, Vera L.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

The mechanisms of toxicity of Ag nanoparticles (NPs) are unclear, in particular in the terrestrial environment. In this study the effects of AgNP (AgNM300K) were assessed in terms of oxidative stress in the soil worm Enchytraeus crypticus, using a range of biochemical markers [catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), total glutathione (TG), metal...


Cellular energy allocation to assess the impact of nanomaterials on soil invert...

Gomes, Susana I. L.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

The effects of several copper (Cu) and silver (Ag) nanomaterials were assessed using the cellular energy allocation (CEA), a methodology used to evaluate the energetic status and which relates with organisms’ overall condition and response to toxic stress. Enchytraeus crypticus (Oligochatea), was exposed to the reproduction effect concentrations EC20/50 of several Cu and Ag materials (CuNO3, Cu-Field, Cu-Nwires...


Cu-nanoparticles ecotoxicity: explored and explained?

Gomes, Susana I. L.; Murphy, Michael; Nielsen, Margrethe T.; Kristiansen, Soren M.; Amorim, Mónica J. B.; Scott-Fordsmand, Janeck J.

The nano-form of copper (Cu-NPs) is already extensively used. In this paper the toxic effect of Cu in the worm Enchytraeus crypticus (Oligochaeta: Enchytraeidae) was assessed following exposure to (1) Cu-salt: freshly spiked soil with copper-nitrate, (2) Cu-NPs: freshly spiked soil with Cu nanoparticles (80 nm), and (3) Cu-field: historically Cu contaminated soil (80 years ago). Our main aims were to compare th...


Enchytraeid Reproduction Test(PLUS): hatching, growth and full life cycle test-...

Bicho, Rita C.; Santos, Fátima C. F.; Gonçalves, Micael F. M.; Soares, Amadeu M. V. M.; Amorim, Mónica J. B.

Soil ecotoxicity standard tests for invertebrates are usually limited to the assessment of endpoints like survival and reproduction. Adverse effects may occur at other developmental stages, e.g. embryo development, hatching or growth. The species Enchytraeus crypticus is a model organism in the standard soil ecotoxicology test, where survival and reproduction are assessed. In the present study we optimized the ...


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