3 documents found, page 1 of 1
Use of the same polymer for synthesis and purification of peptides
Silva,Elias H.; Etchegaray,Augusto; Carvalho,Regina S. H.; Jubilut,Guita N.; Miranda,Antonio; Nakaie,Clovis R.This work reveals an uncommon but valuable biotechnological approach regarding the use of a same polymer (benzhydrylamine-resin, BHAR) for synthesis and anion exchange purification of peptides. Initially, the octapeptide DRVYIHPF-NH2 was synthesized in 1% and 3% cross-linked BHAR, attaching 2.5 mmol g-1 ammonium groups. Due certainly to its less rigid polymeric backbone, higher synthesis yield (about 80%) was a...
Importance of the solvation degree of peptide-resin beads for amine groups dete...
Cilli,Eduardo M.; Jubilut,Guita N.; Ribeiro,Suely C. F.; Oliveira,Eliandre; Nakaie,Clovis R.The classic and important picric acid method used in polymers biochemical and chemical fields of polymers for amine group quantification was chosen in this work as a model for evaluating the influence of the resin bead solvation during an analytical procedure. It was observed that this method, proposed almost three decades ago, failed to quantify amine groups of peptidyl-resin containing aggregating and polar s...
Comparative time-course study of aminoacyl- and dipeptidyl-resin hydrolysis
Jubilut,Guita N.; Marchetto,Reinaldo; Cilli,Eduardo M.; Oliveira,Eliandre; Miranda,Antonio; Tominaga,Mineko; Nakaiee,Clóvis R.The classic hydrolysis procedure for quantification of resin-bound aminoacyl and peptidyl groups with 12 N HCl: propionic acid was reevaluated by studying the influence of the nature of the resin and the resin-bound group. Their stability during acid hydrolysis was dependent on the C-terminal amino acid, and the order of acid stability was Phe > Val > Gly. Otherwise, the dipeptides Ala-Gly, Ala-Val, and Ala-Phe...