4 documents found, page 1 of 1
Dissection of the pre-germinal center B-cell maturation pathway in common varia...
del Pino-Molina, Lucía; López-Granados, Eduardo; Lecrevisse, Quentin; Torres Canizales, Juan; Pérez-Andrés, Martín; Blanco, Elena; Wentink, MarjoleinIntroduction: Common Variable Immunodeficiency (CVID) is characterized by defective antibody production and hypogammaglobulinemia. Flow cytometry immunophenotyping of blood lymphocytes has become of great relevance for the diagnosis and classification of CVID, due to an impaired differentiation of mature post-germinal-center (GC) class-switched memory B-cells (MBC) and severely decreased plasmablast/plasma cell...
The EuroFlow PID orientation tube for flow cytometric diagnostic screening of p...
van der Burg, Mirjam; Kalina, Tomas; Perez-Andres, Martin; Vlkova, Marcela; Lopez-Granados, Eduardo; Blanco, Elena; Bonroy, Carolien; Sousa, Ana E.In the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for all lymphoid PIDs and offer high sensitivity to identify a lymph...
Guidelines for the use of flow cytometry and cell sorting in immunological stud...
Cossarizza, Andrea; Chang, Hyun‐Dong; Radbruch, Andreas; Acs, Andreas; Adam, Dieter; Adam‐Klages, Sabine; Agace, William W.; Aghaeepour, NimaThese guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human...
Age-associated distribution of normal B-cell and plasma cell subsets in periphe...
Blanco, Elena; Pérez-Andrés, Martín; Arriba-Méndez, Sonia; Contreras-Sanfeliciano, Teresa; Criado, Ignacio; Pelak, Ondrej; Serra-Caetano, AnaBackground: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory B-cell (MBC) and plasma cell (PCs) compartments remains to be i...