6 documents found, page 1 of 1
Exosomes modified with anti-MEK1 siRNA lead to an effective silencing of triple...
Ferreira, Débora Carina Gonçalves Abreu; Pereira, Cátia Sofia Santos; Costa, Marta; Afonso, Julieta Alexandra Pereira; Yang, Sujuan; Hensel, JanineTriple negative breast cancer (TNBC) is a highly heterogenous disease not sensitive to endocrine or HER2 therapy and standardized treatment regimens are still missing. Therefore, development of novel TNBC treatment approaches is of utmost relevance. Herein, the potential of MAPK/ERK downregulation by RNAi-based therapeutics in a panel of mesenchymal stem-like TNBC cell lines was uncovered. Our data revealed tha...
Exosome-mediated MEK1 silencing is a promising approach against triple negative...
Ferreira, Débora; Cátia S. Pereira; Costa, Marta; Afonso, Julieta; Longatto, Adhemar; Baltazar, Fátima; Kalluri, Raghu; Moreira, João N.[Excerpt] Introduction: Breast cancer is a major public health problem worldwide being the most diagnosed cancer in women [1] . Triple negative breast cancer (TNBC) represents 10-20% of all breast cancers and is characterized by the absence of hormone receptors (progesterone and estrogen) and lack of expression of epidermal growth factor receptor 2. Furthermore, it is also described to have poor prognosis due t...
Extracellular vesicles from pancreatic cancer stem cells lead an intratumor com...
Ruivo, Carolina F.; Bastos, Nuno; Adem, Barbara; Batista, Ines; Duraes, Cecilia; Melo, Carlos A.; Castaldo, Stephanie A.; Campos‐Laborie, FranciscoObjective: Intratumor heterogeneity drives cancer progression and therapy resistance. However, it has yet to be determined whether and how subpopulations of cancer cells interact and how this interaction affects the tumour. Design: We have studied the spontaneous flow of extracellular vesicles (EVs) between subpopulations of cancer cells: cancer stem cells (CSC) and non-stem cancer cells (NSCC). To determine th...
Engineered exosome-mediated delivery of RNAi therapeutics towards triple negati...
Ferreira, Débora; Costa, Marta; Baltazar, Fátima; Kalluri, Raghu; Moreira, João N.; Rodrigues, L. R.[Excerpt] Breast cancer was the leading cause of global cancer incidence in 2020, with an estimated 2.3 million new diagnosed cases among women. Triple negative breast cancer (TNBC) characterized by the absence of hormone receptors, lack of expression of epidermal growth factor receptor-2 and poor prognosis, represents about 10-20% of all breast cancers. Hence, an efficient targeted delivery system is urgently ...
Peptide-mediated targeted delivery system towards triple negative breast cancer...
Ferreira, Débora; Moreira, João N.; Kalluri, Raghu; Rodrigues, L. R.About 2.1 million new diagnosed breast cancer cases among women were estimated for 2018. Triple negative breast cancer (TNBC), characterized by the absence of hormone receptors (estrogen and progesterone), lack of expression of epidermal growth factor receptor-2 and poor prognosis, represents 10-20% of all breast cancers. Hence, the identification of novel biomarkers for this type of breast cancer is highly rel...
Genome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhan...
Léveillé, Nicolas; Melo, Carlos A.; Rooijers, Koos; Díaz-Lagares, Angel; Melo, Sonia A.; Korkmaz, Gozde; Lopes, Rui; Moqadam, Farhad Akbari; Maia, Ana Rp53 binds enhancers to regulate key target genes. Here, we globally mapped p53-regulated enhancers by looking at enhancer RNA (eRNA) production. Intriguingly, while many p53-induced enhancers contained p53-binding sites, most did not. As long non-coding RNAs (lncRNAs) are prominent regulators of chromatin dynamics, we hypothesized that p53-induced lncRNAs contribute to the activation of enhancers by p53. Among ...