Made available in DSpace on 2019-09-11T20:58:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2007; Considerando a escassez de antígenos quimicamente definidos, realmente úteis e confiáveis para aplicação na soroepidemiologia da esquistossomose em larga escala, foi proposto, neste trabalho, um método alternativo para a seleção de clones de cDNA que expressam proteínas com putativo potencial diagnóstico na e...
Made available in DSpace on 2019-09-11T20:58:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2008; Isolados de Cryptosporidium identificados em quatorze amostras de fezes, coletadas de cinco pacientes com infecção por HIV e de nove crianças imunocompetentes, residentes no estado de São Paulo, Brasil, foram submetidos a análise molecular por Nested-PCR, seguido da caracterização genética por polimorfismo do...
Made available in DSpace on 2019-09-11T20:58:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2002; The high sensitivity and the ability to diagnose schistosomiasis in a very early phase after infection have indicated the detection of IgM antibodies to Schistosoma mansoni gut antigens by the immunofluorescence test (IgM-IFT) as a useful serological test for epidemiological studies in low endemic areas. When...
Made available in DSpace on 2019-09-12T16:26:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2016; This study analyzed the environmental and health aspects related to babaçu coconut breakers belonging to the Regional Association of Rural Female Workers, Tocantins State (Portuguese acronym “ASMUBIP-TO”). The breaking of the babaçu coconut is a painful activity, often performed by women of low income, living...
Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the Sate of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment a...
Considering the scarcity of defined antigens, actually useful and reliable for use in the field studies, we propose an alternative method for selection of cDNA clones with potential use in the diagnosis of schistosomiasis. Human antibodies specific to a protein fraction of 31/32 kDa (Sm31/32), dissociated from immune complexes, are used for screening of clones from an adult worm cDNA library. Partial sequencing...