19 documents found, page 1 of 2
CD34-positive cells and their subpopulations characterized by flow cytometry an...
Carvalho,Jerusa Martins; Souza,Marlon Knabben de; Buccheri,Valéria; Rubens,Cláudia Viviane; Kerbauy,José; Oliveira,José Salvador Rodrigues deCONTEXT AND OBJECTIVE: Counting and separating hematopoietic stem cells from different sources has importance for research and clinical assays. Our aims here were to characterize and quantify hematopoietic cell populations in marrow donors and to evaluate CD34 expression and relate this to engraftment. DESIGN AND SETTING: Cross-sectional study on hematopoietic stem cell assays, using flow cytometry on donor bon...
Proliferating cell nuclear antigen (PCNA), p53 and MDM2 expression in Hodgkins...
Pinheiro,Gevina Silva; Silva,Maria Regina Régis; Rodrigues,Celso Arrais; Kerbauy,José; Oliveira,José Salvador Rodrigues deCONTEXT AND OBJECTIVE: Tumor cells in Hodgkins disease (HD) express cell proliferation markers that are evaluated according to the oncogenes involved or the expression of their proteins. Correlations between the protein expression grade and clinical data are now important for disease prognosis. DESIGN AND SETTING: This was a retrospective analysis on proliferating cell nuclear antigen (PCNA), p53 and MDM2 (mur...
Cariótipo em leucemia mielóide aguda: importância e tipo de alteração em 30 pac...
Pelloso,Luís Arthur Flores; Chauffaille,Maria de Lourdes Lopes Ferrari; Ghaname,Fabiana Sinnot; Yamamoto,Mihoko; Bahia,Daniella Márcia MaranhãoOBJETIVO: Análise sob o ponto de vista citogenético e clínico de pacientes com LMA. MÉTODOS: Foram estudados 30 pacientes, sendo 16 homens e 14 mulheres. A idade variou de 19 a 84 anos. O diagnóstico baseou-se na classificação OMS, imunofenotipagem e citogenética clássica por banda G. Foram tratados com o protocolo daunorrubicina e citarabina (3+7), com adição de ATRA na Leucemia Promielocítica Aguda. RESULTADO...
Myelodysplastic syndromes: histopathology as prognostic factor
Romeo,Maura; Chauffaille,Maria L.F.; Silva,Maria R.R.; Kerbauy,JoséBone marrow biopsy allows evaluation of cellularity, abnormal localization of immature precursors and fibrosis in myelodysplastic syndrome. It has been considered important to make diagnosis and prognosis of this disorder. The object of this study evaluated the influence of histopathological parameters, such as cellularity, erythroid/myeloid ratio, abnormal localization of immature precursors and marrow fibrosi...
Fluorescent in-situ hybridization (FISH) for BCR/ABL in chronic myeloid leukemi...
Chauffaille,Maria de Lourdes Lopes Ferrari; Oliveira,José Salvador Rodrigues; Romeo,Maura; Kerbauy,JoséCONTEXT: Identification of Philadelphia chromosome or BCR/ABL gene rearrangement in chronic myeloid leukemia is important at diagnosis as well as after treatment. OBJECTIVE: To compare the results of karyotyping using fluorescent in-situ hybridization (FISH) upon diagnosis and 1 year after bone marrow transplantation in 12 patients. TYPE OF STUDY: Diagnostic test and residual disease detection. SETTING: Hematol...
Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic ...
Chauffaille,Maria de Lourdes L.F.; Marques,Eliana Azevedo; Oliveira,Jose Salvador Rodrigues de; Rodrigues,Maria Madalena; Figueiredo,Maria StellaChronic lymphocytic leukemia (CLL) presents a varying incidence of karyotypic abnormalities whose detection is complicated by difficulties in obtaining mitosis for analysis in this type of mature lymphocyte disorder. Since the introduction of molecular cytogenetics (FISH = fluorescent in situ hybridization), applying centromeric probes for chromosome 12 has made it possible to detect a higher percentage of tris...
Prognostic factors in non-Hodgkin lymphomas
Cecyn,Karin Zattar; Oliveira,José Salvador Rodrigues de; Alves,Antônio Correia; Silva,Maria Regina Regis; Kerbauy,JoséCONTEXT: In Hodgkin's disease, each clinical or pathologic stage can be related to the extent of the area involved and predicts the next anatomical region at risk for tumor dissemination. OBJECTIVE: To determine the best prognostic factors that could predict survival in non-Hodgkin lymphoma cases. DESIGN: A retrospective study. LOCATION: Department of Hematology and Transfusion Medicine, Universidade Federal de...
Prognosis related to staging systems for chronic lymphocytic leukemia
Faria,José Roberto de; Oliveira,José Salvador Rodrigues de; Faria,Rosa Malena Delbone de; Silva,Maria Regina Regis; Goihman,Samuel; Yamamoto,MiohokoCONTEXT: Chronic lymphocytic leukemia (CLL) is a clonal lymphoproliferative disorder, characterized by B lymphocytic proliferation. CLL is the most frequent adult leukemia in Western countries, accounting for 25 to 30% of all white leukemic patients. OBJECTIVE: To evaluate clinical and staging characteristics in prognosis of chronic lymphocytic leukemia. DESIGN: Evaluation of clinical-staging data. SETTING: Uni...
Clinical management of six cases of low-risk primary tonsillar non-Hodgkin´s ly...
Colleoni,Gisele Wally Braga; Oliveira,José Salvador Rodrigues; Alves,Antonio Correa; Borducchi,Davimar Miranda Maciel; Segreto,Roberto AraújoCONTEXT: There have been many reports that favor aggressive systemic treatment with chemotherapy and radiotherapy, even for well-localized lymphomas, avoiding the need for tonsillectomy of the normal tonsil. CASE REPORT: We report six cases of primary tonsillar lymphoma with a median patient age of 42 years. There were two lymphoma cases with diffuse large cells, two cases with mixed small and large cells, one ...
Langerhans cell histiocytosis
Chauffaille,Maria de Lourdes L. F.; Valério,Rosana M.; Diniz,Cybelle Maria Costa; Simões,Milvia Maria; Enokihara,Silva; Michalany,NylceoThe authors present a rare case of Langerhans cell histiocytosis in a 31 year old female patient with vulvar, peri-anal and oral lesions, diabetes insipidus, pulmonary skin and bone infiltrations. Skin biopsy immunohistochemistry presented positive S100 protein and vimentine, but the diagnosis was done with the demonstration of Birbeck granules with eletronic mucroscopy. The treatment was based on systemical ch...